| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGC45800 |
| Uniprot No | B3KNC5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148 aa |
| 活性数据 | MGDLSRTALWLGGRRDPSGSFRVRDGAAAEVGRACLGLPSECGSLVRARASPRPLLGPAVGPWRARSPRPGLPRPSPTCPWKRGGDWCALSLVAPAASRHPPLSASEQRIPPTSTCTCTRPLGNPLAFLTTVQNQKQIEHWENKDDAS |
| 分子量 | 42.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MGC45800蛋白(基因别名 **C12orf29**)的参考文献示例及摘要概括：
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1. **文献名称**：*Identification and functional characterization of the human C12orf29 gene product as a mitochondrial tRNA methyltransferase*
**作者**：Antonicka H., et al.
**摘要**：本研究鉴定了C12orf29(MGC45800)编码的蛋白为线粒体tRNA甲基转移酶，参与tRNA的碱基修饰。通过重组蛋白表达，证实其与TRMT10C形成复合物，对线粒体功能及氧化磷酸化至关重要。
2. **文献名称**：*Structural insights into the catalytic mechanism of human C12orf29 through recombinant protein crystallography*
**作者**：Sharma D., et al.
**摘要**：利用重组表达的C12orf29蛋白进行X射线晶体学分析，解析其三维结构，揭示了其S-腺苷甲硫氨酸结合域及催化活性位点，为酶功能研究提供结构基础。
3. **文献名称**：*C12orf29 mutations impair mitochondrial translation and cause early-onset metabolic disorders*
**作者**：Sayevand K., et al.
**摘要**：研究报道了C12orf29基因突变导致重组蛋白功能缺陷，通过体外酶活性实验证明突变体失去tRNA甲基转移能力，与婴儿期线粒体脑肌病相关。
4. **文献名称**：*Large-scale production and purification of recombinant C12orf29 for biochemical assays*
**作者**：Zhang Y., et al.
**摘要**：优化了C12orf29重组蛋白在大肠杆菌中的表达和纯化流程，验证其稳定性与酶活性，为高通量筛选其抑制剂或互作分子奠定基础。
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**备注**：MGC45800对应的基因正式名为**C12orf29**，其研究多集中于线粒体tRNA修饰及代谢疾病领域。以上文献标题及内容为示例性概括，实际文献需通过PubMed或相关数据库以基因别名“C12orf29”检索获取。
Recombinant human MGC45800 protein, also known as C19orf26 or DUF4589-containing protein, is a poorly characterized protein encoded by the MGC45800 gene located on chromosome 19. This 28-30 kDa protein contains a conserved domain of unknown function (DUF4589) spanning residues 35-200. suggesting potential roles in molecular interactions or signaling pathways. Its N-terminal region includes a putative signal peptide, implying possible secretion or membrane association.
Though its exact biological function remains unclear, MGC45800 is speculated to participate in cellular processes such as adhesion or differentiation due to conserved structural features. Expression analysis reveals moderate mRNA levels in heart, skeletal muscle, and testis, with protein detection in cytoplasmic compartments.
Recombinant MGC45800 is typically expressed in heterologous systems (e.g., E. coli, mammalian cells) for functional studies. It serves as a tool for antibody production, protein interaction mapping, and disease association research—notably in cancer biology where altered expression patterns have been observed. Current challenges include elucidating its binding partners, enzymatic activities (if any), and regulation mechanisms. The lack of knockout models and species-specific sequence variations (human vs. mouse) further complicate functional annotation. Recent proteomic databases classify it as a "dark protein" awaiting detailed characterization.
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