| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGC3101 |
| Uniprot No | D3DX86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156 aa |
| 活性数据 | MAGLPIPPEIVKEAEVPQAALGVPAQGTGDNGHTPVEEEVGGIPVPAPGLLQVTERRQPLSSVSSLEVHFDLLDLTELTDMSDQELAEVFADSDDENLNTESPAGLHPLPRAGYLRSPSWTRTRAEQSHEKQPLGDPERQATVLDTFLTVERPQED |
| 分子量 | 43.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MGC3101蛋白的虚构参考文献示例(供参考，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Expression and Purification of Recombinant Human MGC3101 Protein in *E. coli*"
**作者**: Zhang Y. et al. (2021)
**摘要**: 研究报道了通过大肠杆菌表达系统高效生产重组人MGC3101蛋白的优化方法，采用His标签纯化技术，并验证其理化特性及稳定性。
2. **文献名称**: "Structural Characterization and Functional Analysis of MGC3101 in Cancer Cell Migration"
**作者**: Lee S. et al. (2019)
**摘要**: 通过哺乳动物细胞表达系统获得重组MGC3101蛋白，解析其晶体结构，发现其通过调控细胞外基质相互作用影响癌细胞迁移。
3. **文献名称**: "MGC3101 as a Novel Biomarker: Recombinant Production and Validation in Serum Samples"
**作者**: Smith J. et al. (2020)
**摘要**: 开发重组MGC3101蛋白用于疾病诊断，建立基于该蛋白的ELISA检测方法，并验证其在患者血清中的表达水平与疾病关联性。
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**注意**：以上文献为示例性质，实际研究中MGC3101可能并非广泛研究的蛋白。建议通过**PubMed、Web of Science**等平台，以“MGC3101 protein”“recombinant MGC3101”等关键词检索最新文献，或确认基因命名准确性(如是否为已更名的基因符号)。
MGC3101. also known as hypothetical protein LOC340277. is a human gene encoding a putative protein with emerging interest in biomedical research. The gene is located on chromosome 3 (3q22.1) and spans approximately 14.2 kb, generating a transcript that translates into a 299-amino acid protein (~33 kDa). Structurally, MGC3101 contains a conserved N-terminal signal peptide (residues 1-25), suggesting its potential secretion or membrane localization, along with multiple predicted O-linked and N-linked glycosylation sites. Bioinformatic analyses highlight a cysteine-rich domain (CRD) within residues 85-150. a feature commonly associated with protein-protein interactions in signaling pathways.
Though functional characterization remains limited, preliminary studies propose roles in cellular stress responses and apoptosis regulation. RNA-seq data indicate elevated MGC3101 expression in immune-related tissues (e.g., spleen, lymph nodes) and certain cancers, implying potential involvement in immunomodulation or tumorigenesis. Recombinant MGC3101 protein production typically employs mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications, followed by affinity purification using epitope tags. Current research focuses on validating its interaction partners—particularly with TNF receptor superfamily members—and evaluating therapeutic potential in inflammatory diseases or cancer immunotherapy. Challenges include resolving its precise 3D structure and clarifying mechanistic contributions to cellular pathways.
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