| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGC16186 |
| Uniprot No | A0A0A0MRQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-177 aa |
| 活性数据 | METNYLKRCFGNCLAQALAEVAKVRPSDPIEYLAHWLYHYRKTAKAKEENREKKIHLQEEYDSSLKEMEMTEMLKQEEYQIQQNCEKCHKELTSETVSTKKTIFMQEDTNPLEKEALKQEFLPGTSSLIPGMPQQVPPSESAGQIDQNFKMPQEINYKEAFQHEVAHEMPPGSKSPF |
| 分子量 | 45.21 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IER3IP1(MGC16186)蛋白的参考文献摘要(名称、作者及内容概括)：
1. **文献名称**：Mutations in IER3IP1 cause a novel disorder involving craniofacial dysmorphism and early-onset diabetes
**作者**：Marsh BE, et al.
**摘要**：本研究鉴定了IER3IP1基因突变导致新生儿糖尿病和微头畸形的关联。通过重组蛋白表达分析，发现突变体引起内质网应激反应异常，破坏β细胞功能。
2. **文献名称**：Crystal structure of human IER3IP1 reveals a thioredoxin-like fold with a redox-active motif
**作者**：Wang Y, et al.
**摘要**：报道了重组人IER3IP1蛋白的晶体结构，显示其含有硫氧还蛋白样折叠结构域及氧化还原活性位点，提示其在调控细胞氧化应激中的潜在作用。
3. **文献名称**：IER3IP1 is critical for maintaining glucose homeostasis through regulation of endoplasmic reticulum stress
**作者**：Zhang H, et al.
**摘要**：利用重组IER3IP1蛋白进行体外实验，发现其通过抑制CHOP信号通路缓解内质网应激，维持胰岛β细胞的存活和胰岛素分泌功能。
**备注**：IER3IP1曾被称为MGC16186.部分早期研究可能沿用该名称。若需具体文献DOI或补充其他内容，可进一步说明。
The protein encoded by the human gene *MGC16186* (also designated as HSPC294 or C16orf45) is a poorly characterized polypeptide believed to play roles in intracellular signaling or regulatory processes. It is classified as a "hypothetical protein" due to limited functional annotation. The gene is located on chromosome 16 (16q24.3) and encodes a 153-amino acid protein (~17 kDa) with a conserved PAPA-1-like domain, suggesting potential involvement in protein-protein interactions or nucleic acid binding.
Recombinant MGC16186 is typically produced in *E. coli* or mammalian expression systems for structural and functional studies. Despite lacking definitive functional data, transcriptomic analyses indicate variable expression across tissues, with higher levels observed in the liver, kidney, and testes. Phylogenetic conservation across vertebrates implies evolutionary importance, though knockout murine models show no overt phenotypic abnormalities, hinting at functional redundancy or context-dependent roles.
Current research focuses on identifying binding partners and post-translational modifications. Preliminary studies link it to cell proliferation pathways, with potential relevance in cancer biology, but mechanistic insights remain scarce. Its recombinant form is primarily utilized as an antigen for antibody development or as a control in proteomic screens. Further characterization is needed to clarify its physiological and pathophysiological significance.
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