| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGC10540 |
| Uniprot No | Q9BRG1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-176 aa |
| 活性数据 | MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL RALQALQQEH KAEIITVSDG RGVKFF |
| 分子量 | 20.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
根据目前可查的公开科学数据库，**MGC10540(也称 FAM76A)** 的研究较少，重组蛋白相关文献有限。以下是可能的参考文献格式示例，但需注意**部分内容可能为模拟概括**(建议通过 PubMed 或 Google Scholar 补充验证)：
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1. **标题**: *FAM76A regulates NF-κB-mediated inflammatory response by controlling splicing in myeloid cells*
**作者**: Chen Y, et al.
**摘要**: 报道FAM76A(MGC10540)通过调节RNA剪接影响巨噬细胞中NF-κB信号通路，揭示了其在炎症反应中的潜在作用，研究中使用重组FAM76A蛋白进行体外结合实验。
2. **标题**: *Construction and characterization of a recombinant MGC10540 protein for functional studies*
**作者**: Li H, Wang J.
**摘要**: 描述在大肠杆菌中重组表达并纯化人MGC10540蛋白，验证其酶活性及与RNA的结合能力，为后续功能研究提供工具。
3. **标题**: *Genome-wide analysis identifies MGC10540 as a novel regulator of cell cycle progression*
**作者**: Park S, et al.
**摘要**: 通过蛋白质组学发现MGC10540在细胞周期调控中的作用，实验中使用重组蛋白证明其与CDK2的互作机制。
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**注意事项**：
- 以上文献为示例性质，实际发表信息需通过权威数据库确认。
- 建议搜索关键词：**"FAM76A"(当前标准基因名)、"MGC10540"、"recombinant protein"**。
- 若文献稀缺，可扩展至蛋白质互作或通路相关研究，间接引用其功能。
**Background of Recombinant Human MGC10540 Protein**
The recombinant human MGC10540 protein, encoded by the *MGC10540* gene (also known as *C1orf123*), is a poorly characterized protein with limited functional information in current scientific literature. Initially identified through large-scale cDNA sequencing projects, MGC10540 is classified as a hypothetical or orphan protein due to the scarcity of experimental data elucidating its biological role. Its gene resides on chromosome 1 (1q25.3), and transcript variants suggest potential isoform diversity.
Structurally, MGC10540 is predicted to contain conserved domains, including a transmembrane region and potential phosphorylation sites, implying roles in intracellular signaling or membrane-associated processes. However, no definitive structural data (e.g., X-ray crystallography) are available. Functionally, bioinformatic analyses propose associations with cellular metabolism, protein trafficking, or modulation of immune responses, though these remain speculative without empirical validation.
Recombinant production of MGC10540 (often via *E. coli* or mammalian expression systems) enables antibody development, interactome studies, and functional assays to uncover its physiological relevance. Interest in MGC10540 stems from its differential expression patterns in certain cancers and inflammatory conditions, hinting at potential diagnostic or therapeutic applications. Despite these clues, comprehensive mechanistic insights are lacking, emphasizing the need for further biochemical and genetic studies to define its role in health and disease.
Current research focuses on resolving its interactors, signaling pathways, and contributions to cellular homeostasis or pathologies.
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