| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MGAT4B |
| Uniprot No | Q9UQ53 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-563 aa |
| 活性数据 | MSRVAGTRTDVNELLQRWTPRCVRWHTGGARRVALDRPLVTACLPPAGDVVDVYQREFLALRDRLHAAEQESLKRSKELNLVLDEIKRAVSERQALRDGDGNRTWGRLTEDPRLKPWNGSHRHVLHLPTVFHHLPHLLAKESSLQPAVRVGQGRTGVSVVMGIPSVRREVHSYLTDTLHSLISELSPQEKEDSVIVVLIAETDSQYTSAVTENIKALFPTEIHSGLLEVISPSPHFYPDFSRLRESFGDPKERVRWRTKQNLDYCFLMMYAQSKGIYYVQLEDDIVAKPNYLSTMKNFALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMFYRDKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKQALRKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDKLFNTSVEVLPFDNPQSDKEALQEGRTATLRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTDSPVWVILSEIFLKKAD |
| 分子量 | 91.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MGAT4B蛋白的3篇虚构参考文献示例，涵盖不同研究方向，供参考：
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1. **文献名称**：*Recombinant Expression of Human MGAT4B in HEK293 Cells and Its Role in N-Glycan Branching*
**作者**：Chen, L. et al.
**摘要**：本研究通过HEK293细胞成功表达了重组人MGAT4B蛋白，并验证其β-1.4-N-乙酰氨基葡萄糖转移酶活性。实验表明，MGAT4B通过催化N-糖链的β1-4分支合成，影响细胞表面整合素的糖基化修饰，进而调节细胞黏附与迁移能力。
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2. **文献名称**：*Structural Insights into MGAT4B Substrate Specificity via Cryo-EM Analysis*
**作者**：Kumar, S. et al.
**摘要**：利用冷冻电镜技术解析了重组MGAT4B蛋白与UDP-GlcNAc底物的复合物结构，揭示了其底物结合口袋的关键氨基酸残基。研究为开发靶向MGAT4B的小分子抑制剂提供了结构基础，可能用于治疗因MGAT4B过表达导致的癌症转移。
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3. **文献名称**：*MGAT4B Knockout Mice Reveal Its Critical Role in Autoimmune Regulation*
**作者**：Tanaka, M. et al.
**摘要**：通过构建MGAT4B基因敲除小鼠模型，发现其缺失导致T细胞受体(TCR)的异常糖基化，从而抑制自身免疫性关节炎的发展。研究提示靶向MGAT4B或可成为自身免疫疾病的新型治疗策略。
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注：上述文献为示例，实际研究中请以真实存在的文献为准。建议通过PubMed或Web of Science等数据库，结合关键词“MGAT4B”、“recombinant expression”或“glycosyltransferase”进行检索。
MGAT4B (Mannosyl (Alpha-1.3-)-Glycoprotein Beta-1.4-N-Acetylglucosaminyltransferase, Isozyme B) is a key glycosyltransferase enzyme involved in N-glycan biosynthesis. It catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the α-1.3-mannose branch of hybrid and complex glycoproteins, forming β-1.4 linkages that influence glycan branching patterns. This post-translational modification critically affects glycoprotein stability, cell-cell communication, and immune recognition. The MGAT4B gene is located on chromosome 5q35 in humans and is regulated by multiple transcription factors, including Sp1 and Ets family proteins.
Aberrant MGAT4B expression has been linked to disease pathogenesis. Elevated MGAT4B activity increases β-1.6 branching in cancer cells, promoting tumor metastasis through enhanced integrin-mediated cell motility and matrix adhesion. Conversely, MGAT4B deficiency in mice leads to autoimmune-like symptoms, suggesting its role in immune tolerance. Recombinant human MGAT4B protein, typically produced in mammalian expression systems, retains enzymatic activity for in vitro studies of glycan remodeling. Its crystal structure reveals a conserved GT-A fold with a DXD motif essential for Mn²⁺ cofactor binding. Current research focuses on developing MGAT4B inhibitors as potential anticancer agents and using recombinant protein to modulate glycosylation in biotherapeutic production.
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