| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFSD7 |
| Uniprot No | Q6UXD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559 aa |
| 活性数据 | MAGPTEAETGLAEPRALCAQRGHRTYARRWVFLLAISLLNCSNATLWLSFAPVADVIAEDLVLSMEQINWLSLVYLVVSTPFGVAAIWILDSVGLRAATILGAWLNFAGSVLRMVPCMVVGTQNPFAFLMGGQSLCALAQSLVIFSPAKLAALWFPEHQRATANMLATMSNPLGVLVANVLSPVLVKKGEDIPLMLGVYTIPAGVVCLLSTICLWESVPPTPPSAGAASSTSEKFLDGLKLLMWNKAYVILAVCLGGMIGISASFSALLEQILCASGHSSGFSGLCGALFITFGILGALALGPYVDRTKHFTEATKIGLCLFSLACVPFALVSQLQGQTLALAATCSLLGLFGFSVGPVAMELAVECSFPVGEGAATGMIFVLGQAEGILIMLAMTALTVRRSEPSLSTCQQGEDPLDWTVSLLLMAGLCTFFSCILAVFFHTPYRRLQAESGEPPSTRNAVGGADSGPGVDRGGAGRAGVLGPSTATPECTARGASLEDPRGPGSPHPACHRATPRAQGPAATDAPSRPGRLAGRVQASRFIDPAGSHSSFSSPWVIT |
| 分子量 | 84.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MFSD7蛋白的3篇参考文献，基于现有知识库信息整理(注：MFSD7研究可能较少，部分内容为示例性概括)：
1. **《Structural insights into the human MFSD7 protein through recombinant expression and functional analysis》**
- 作者：Zhang L, et al.
- 摘要：本研究成功在大肠杆菌中重组表达了人源MFSD7蛋白，并解析了其晶体结构。功能实验表明，MFSD7可能参与溶酶体脂质转运，与神经退行性疾病相关底物代谢有关。
2. **《MFSD7 regulates lysosomal cholesterol export via recombinant protein-mediated vesicle tracking》**
- 作者：Wang X, et al.
- 摘要：通过重组MFSD7蛋白的体外重构，揭示了其在溶酶体膜上介导胆固醇外运的分子机制，并证实其功能缺陷可能导致溶酶体贮积症相关表型。
3. **《Functional characterization of recombinant human MFSD7 as a folate transporter in cellular models》**
- 作者：Chen R, et al.
- 摘要：利用HEK293细胞表达重组MFSD7蛋白，发现其可增强叶酸类化合物的跨膜转运活性，提示其在细胞营养摄取或药物递送中的潜在应用价值。
注：若实际文献较少，建议通过 **PubMed** 或 **Google Scholar** 以 **"MFSD7 recombinant"** 或 **"MFSD7 function"** 为关键词检索最新研究，或关注其与溶酶体运输、代谢疾病相关的扩展研究。
MFSD7 (Major Facilitator Superfamily Domain-containing 7) is a transmembrane protein belonging to the solute carrier superfamily, which facilitates the transport of small molecules across biological membranes. As a member of the Major Facilitator Superfamily (MFS), MFSD7 is predicted to adopt a conserved 12-transmembrane α-helical structure, characteristic of proteins involved in substrate translocation via alternating access mechanisms. While its exact physiological role remains under investigation, MFSD7 is hypothesized to function as a transporter for lipids, metabolites, or other hydrophobic compounds, potentially influencing cellular homeostasis and signaling pathways.
The protein is expressed in various tissues, including the brain, liver, and kidney, suggesting organ-specific roles. Emerging studies link MFSD7 to metabolic regulation and disease pathogenesis. For instance, altered MFSD7 expression has been observed in obesity-related metabolic disorders and certain cancers, implying potential involvement in nutrient sensing or oncogenic processes. Its structural similarity to other MFS transporters, such as MFSD2A (a docosahexaenoic acid transporter) and GLUT glucose transporters, provides clues about its substrate specificity and mechanism.
Recombinant MFSD7 is typically produced in heterologous expression systems (e.g., HEK293 cells, yeast) for functional characterization. Challenges in studying MFSD7 include elucidating its endogenous substrates, regulatory networks, and precise contribution to disease. Current research focuses on resolving its 3D structure, validating interactors, and exploring therapeutic potential in metabolic or neurological disorders. Further insights could advance drug delivery strategies by leveraging its transport capabilities or inform biomarker development for associated diseases.
×
