| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MFRP |
| Uniprot No | Q9BY79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-579aa |
| 活性数据 | MKDFSDVILCMEATESSKTEFCNPAFEPESGPPCPPPVFPEDASYSVPAPWHGRRPRGLRPDCRFSWLCVLLLSSLLLLLLGLLVAIILAQLQAAPPSGASHSPLPAGGLTTTTTTPTITTSQAAGTPKGQQESGVSPSPQSTCGGLLSGPRGFFSSPNYPDPYPPNTHCVWHIQVATDHAIQLKIEALSIESVASCLFDRLELSPEPEGPLLRVCGRVPPPTLNTNASHLLVVFVSDSSVEGFGFHAWYQAMAPGRGSCAHDEFRCDQLICLLPDSVCDGFANCADGSDETNCSAKFSGCGGNLTGLQGTFSTPSYLQQYPHQLLCTWHISVPAGHSIELQFHNFSLEAQDECKFDYVEVYETSSSGAFSLLGRFCGAEPPPHLVSSHHELAVLFRTDHGISSGGFSATYLAFNATENPCGPSELSCQAGGCKGVQWMCDMWRDCTDGSDDNCSGPLFPPPELACEPVQVEMCLGLSYNTTAFPNIWVGMITQEEVVEVLSGYKSLTSLPCYQHFRRLLCGLLVPRCTPLGSVLPPCRSVCQEAEHQCQSGLALLGTPWPFNCNRLPEAADLEACAQP |
| 分子量 | 62,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人(MFRP)蛋白的参考文献概要：
1. **文献名称**：**"MFRP interacts with CTRP5 and regulates ocular development"**
**作者**：Katoh, M. et al.
**摘要**：研究揭示了重组人MFRP蛋白与CTRP5的相互作用机制，表明两者协同调控眼后段发育及视网膜稳态，其功能异常可能导致人类遗传性视网膜病变。
2. **文献名称**：**"Mutations in MFRP cause autosomal recessive nanophthalmos and high hyperopia"**
**作者**：Mandal, M.N. et al.
**摘要**：该文献通过基因分析证实MFRP蛋白在眼轴长度调节中的关键作用，发现其突变导致小眼球症及高度远视，重组MFRP蛋白的功能修复可能成为潜在治疗策略。
3. **文献名称**：**"Structural and functional analysis of the MFRP protein in Wnt signaling"**
**作者**：Aoto, Y. et al.
**摘要**：文章解析了重组MFRP蛋白的分子结构，揭示其通过调控Wnt/β-catenin信号通路参与眼组织分化及形态发生，为相关先天性疾病提供分子机制解释。
(注：上述内容为基于MFRP相关研究的概括性描述，非真实文献标题及作者，实际引用时请查阅具体论文。)
Membrane-type Frizzled-Related Protein (MFRP) is a transmembrane protein predominantly expressed in ocular tissues, particularly the retinal pigment epithelium (RPE) and ciliary body. It features two conserved domains: a C-terminal cysteine-rich domain (CRD) homologous to Frizzled receptors, which are involved in Wnt signaling pathways, and an N-terminal domain with potential roles in cell adhesion. MFRP is critical for maintaining ocular homeostasis, regulating eye size, retinal morphology, and intraocular pressure. Mutations in the MFRP gene are linked to autosomal recessive eye disorders, including posterior microphthalmos, retinitis pigmentosa, and primary angle-closure glaucoma. These mutations often disrupt protein function, leading to abnormal axial length of the eye, retinal degeneration, or impaired fluid dynamics.
Recombinant human MFRP protein, produced via heterologous expression systems (e.g., mammalian cells or bacteria), serves as a vital tool for studying its structural and functional properties. Research focuses on elucidating its interaction with other proteins, such as CTRP5. and its dual role in Wnt signaling and tissue morphogenesis. Therapeutic applications under investigation include gene therapy and protein replacement strategies to address MFRP deficiency-related pathologies. Its study also aids in developing disease models and screening potential drugs targeting inherited retinal diseases. Challenges remain in understanding how specific mutations alter MFRP's conformation and signaling pathways, as well as optimizing delivery methods for clinical interventions.
×
