| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MESDC2 |
| Uniprot No | Q14696 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-234aa |
| 活性数据 | MAASRWARKAVVLLCASDLLLLLLLLPPPGSCAAEGSPGTPDESTPPPRKKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPVDFSKIDPSKPESILKMTKKGKTLMMFVTVSGSPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAIFMLRDGSYAWEIKDFLVGQDRCADVTLEGQVYPGKGGGSKEKNKTKQDKGKKKKEGDLKSRSSKEENRAGNKREDL |
| 分子量 | 52.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇模拟的关于重组人MESDC2蛋白的研究文献示例(注：内容为虚构，仅供格式参考)：
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1. **文献名称**: *Recombinant Human MESDC2 Facilitates Wnt Protein Secretion through Chaperone Activity*
**作者**: Takada S. et al.
**摘要**: 研究证明重组表达的MESDC2蛋白在哺乳动物细胞中发挥分子伴侣功能，通过结合Wnt蛋白的保守结构域，调控其内质网到细胞膜的正确运输，从而激活经典Wnt信号通路。
2. **文献名称**: *Expression and Functional Analysis of MESDC2 in Stem Cell Differentiation*
**作者**: Coonrod S.A. et al.
**摘要**: 利用大肠杆菌系统成功表达重组MESDC2蛋白，并发现其通过增强Wnt/β-catenin信号通路活性，促进小鼠胚胎干细胞向中胚层细胞分化，揭示了其在早期发育中的关键作用。
3. **文献名称**: *Structural Insights into MESDC2 as a Therapeutic Target for Cancer*
**作者**: Poe J.C. & Cheng X.
**摘要**: 通过X射线晶体学解析重组MESDC2的三维结构，发现其结合Wnt蛋白的特定口袋区域，抑制其过度分泌可降低多种癌细胞增殖，为靶向治疗提供了理论依据。
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**说明**：以上文献为示例性内容，实际研究中建议通过PubMed、Google Scholar等平台检索关键词(如 "recombinant MESDC2" "MESD2 Wnt")获取真实文献。MESDC2/MESD2的相关研究多聚焦于其作为Wnt信号通路伴侣蛋白的机制及在发育与疾病中的应用。
Recombinant human MESDC2 (Mesoderm Development Candidate 2) protein is a engineered form of the naturally occurring MESDC2. which plays crucial roles in early embryonic development and cellular signaling. MESDC2. also known as Mesd, was initially identified through its essential function in the proper folding and trafficking of low-density lipoprotein receptor-related proteins (LRP5 and LRP6), key components of the Wnt/β-catenin signaling pathway. This pathway is vital for mesoderm formation, cell differentiation, and tissue homeostasis.
Structurally, MESDC2 contains a conserved N-terminal signal peptide and a C-terminal chaperone domain. As a specialized chaperone, it ensures the conformational maturation of LRP5/6 receptors in the endoplasmic reticulum, enabling their cell surface expression and functionality. Disruption of MESDC2 in animal models leads to embryonic lethality due to impaired Wnt signaling and mesodermal defects.
The recombinant version, produced via heterologous expression systems like Escherichia coli or mammalian cells, retains the chaperone activity of native MESDC2. It serves as a valuable tool for studying Wnt signaling mechanisms, receptor-ligand interactions, and cellular differentiation processes. Recent studies also explore its potential therapeutic applications in bone disorders, cancer (where Wnt signaling is often dysregulated), and regenerative medicine. Purified recombinant MESDC2 is increasingly used in biochemical assays, structural studies, and as a reagent to enhance LRP-mediated signaling in experimental models.
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