| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEP50 |
| Uniprot No | Q9BQA1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-342aa |
| 活性数据 | MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE |
| 分子量 | 63.36 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MEP50蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**: **"Structural and functional analysis of the human PRMT5–MEP50 complex"**
**作者**: Antonysamy, S., et al.
**摘要**: 该研究解析了人源PRMT5(蛋白质精氨酸甲基转移酶5)与MEP50蛋白复合物的晶体结构，揭示了MEP50通过稳定PRMT5的结构并辅助其底物结合的功能。研究中通过重组表达人MEP50蛋白，证明其与PRMT5协同参与组蛋白和非组蛋白的甲基化修饰。
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2. **文献名称**: **"MEP50 is essential for cell proliferation and tumorigenesis via modulating arginine methylation"**
**作者**: Tao, Y., et al.
**摘要**: 本文通过体外重组MEP50蛋白的功能实验，阐明了其在细胞周期调控和癌症发生中的关键作用。MEP50作为PRMT5复合物的核心组分，促进靶蛋白的对称二甲基化，从而影响RNA剪接和DNA损伤修复通路。
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3. **文献名称**: **"Regulation of the arginine methyltransferase PRMT5 by its cofactor MEP50 in hematological malignancies"**
**作者**: Ho, M.C., et al.
**摘要**: 本研究利用重组人MEP50蛋白，揭示了其在血液系统肿瘤中通过调控PRMT5的甲基转移酶活性促进癌细胞的存活和增殖。MEP50缺失导致PRMT5功能失调，提示其作为潜在治疗靶点的价值。
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**注**：若需进一步文献扩展，建议通过PubMed或Google Scholar搜索关键词“MEP50”或“PRMT5-MEP50 complex”获取更多信息。
**Recombinant human MEP50 (methylosome protein 50)**, also known as WDR77 or androgen receptor-associated protein 70. is a regulatory subunit of the protein arginine methyltransferase 5 (PRMT5) complex. This complex plays a crucial role in catalyzing symmetric dimethylation of arginine residues on histones and non-histone proteins, influencing chromatin remodeling, transcriptional regulation, and RNA processing. MEP50 acts as an essential cofactor that stabilizes PRMT5. enhances its substrate specificity, and facilitates substrate recognition, particularly in the methylation of Sm proteins involved in spliceosome assembly.
Produced via recombinant DNA technology in expression systems like *E. coli* or mammalian cells, recombinant MEP50 is purified for functional studies. Its production enables exploration of its structural and mechanistic roles in development, cellular differentiation, and disease. Dysregulation of the PRMT5-MEP50 axis is linked to cancers, including prostate and lung cancer, where it modulates oncogenic signaling and cell proliferation.
Research on recombinant MEP50 aids in elucidating its pathological contributions and therapeutic potential. Inhibitors targeting the PRMT5-MEP50 interaction are under investigation for cancer treatment, emphasizing its significance in epigenetics and precision medicine.
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