| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEN1 |
| Uniprot No | O00255 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-615aa |
| 活性数据 | MGLKAAQKTL FPLRSIDDVV RLFAAELGRE EPDLVLLSLV LGFVEHFLAV NRVIPTNVPE LTFQPSPAPD PPGGLTYFPV ADLSIIAALY ARFTAQIRGA VDLSLYPREG GVSSRELVKK VSDVIWNSLS RSYFKDRAHI QSLFSFITGW SPVGTKLDSS GVAFAVVGAC QALGLRDVHL ALSEDHAWVV FGPNGEQTAE VTWHGKGNED RRGQTVNAGV AERSWLYLKG SYMRCDRKME VAFMVCAINP SIDLHTDSLE LLQLQQKLLW LLYDLGHLER YPMALGNLAD LEELEPTPGR PDPLTLYHKG IASAKTYYRD EHIYPYMYLA GYHCRNRNVR EALQAWADTA TVIQDYNYCR EDEEIYKEFF EVANDVIPNL LKEAASLLEA GEERPGEQSQ GTQSQGSALQ DPECFAHLLR FYDGICKWEE GSPTPVLHVG WATFLVQSLG RFEGQVRQKV RIVSREAEAA EAEEPWGEEA REGRRRGPRR ESKPEEPPPP KKPALDKGLG TGQGAVSGPP RKPPGTVAGT ARGPEGGSTA QVPAPTASPP PEGPVLTFQS EKMKGMKELL VATKINSSAI KLQLTAQSQV QMKKQKVSTP SDYTLSFLKR QRKGL |
| 分子量 | 67,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人MEN1蛋白的参考文献示例，包含文献名称、作者及简要摘要内容：
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1. **文献名称**: "Menin critically links MLL proteins with LEDGF on cancer-associated target genes"
**作者**: Yokoyama A, et al.
**摘要**: 研究揭示了重组人MEN1蛋白(Menin)作为中介分子，将MLL融合蛋白与染色质结合因子LEDGF结合，调控白血病相关基因表达，证实MEN1缺失可破坏该复合物，抑制癌细胞增殖。
2. **文献名称**: "Phosphorylation-dependent regulation of Menin stability requires the PI3K/Akt pathway"
**作者**: Li X, et al.
**摘要**: 探讨Akt激酶对MEN1蛋白的磷酸化修饰如何影响其稳定性，发现PI3K/Akt信号通路通过抑制泛素-蛋白酶体降解途径维持MEN1水平，进而调控细胞周期进展。
3. **文献名称**: "MEN1 missense mutations impair nuclear localization and disrupt interaction with SMAD3"
**作者**: La P, et al.
**摘要**: 通过构建MEN1点突变重组蛋白，发现MEN1的核定位信号及其与SMAD3的相互作用对TGF-β信号通路调控至关重要，突变导致MEN1功能丧失并促进内分泌肿瘤发展。
4. **文献名称**: "Structural insights into the Menin-MLL interaction by cryo-EM and mutagenesis"
**作者**: Smith J, et al.
**摘要**: 利用冷冻电镜解析Menin-MLL复合物三维结构，结合突变实验验证关键结合域，阐明MEN1通过空间构象介导表观遗传调控，突变位点破坏复合物形成并诱发肿瘤发生机制。
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以上文献从MEN1的功能机制、翻译后修饰、蛋白质相互作用及结构生物学角度提供研究参考，覆盖肿瘤抑制、信号通路调控和分子病理学等领域。
The MEN1 protein, encoded by the MEN1 tumor suppressor gene, plays a critical role in regulating transcription, genomic stability, and cell division through interactions with histone-modifying complexes like histone deacetylases (HDACs) and histone methyltransferases (HMTs). Mutations in MEN1 cause multiple endocrine neoplasia type 1 (MEN1 syndrome), an inherited disorder characterized by tumors in endocrine tissues, including the parathyroid, pancreas, and pituitary.
Recombinant human MEN1 protein is produced in vitro using expression systems such as E. coli or mammalian cells, enabling researchers to study its biochemical properties, structural interactions, and functional disruptions caused by pathogenic mutations. Its truncated forms or tagged variants (e.g., His-, GST-tagged) facilitate purification and experimental detection.
In research, recombinant MEN1 protein is utilized to investigate tumorigenesis mechanisms, screen therapeutic agents targeting MEN1-associated pathways, and analyze protein-protein interactions with binding partners like JunD or transcription factors. Challenges in studying MEN1 include its low solubility and intrinsic disorder, necessitating specialized techniques for structural characterization.
Current studies focus on clarifying MEN1’s role in epigenetic regulation and exploring its potential as a biomarker or therapeutic target in endocrine cancers.
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