| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MEF2C |
| Uniprot No | Q06413 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 活性数据 | MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVETLRKKGLNGCDSPDPDADDSVGHSPESEDKYRKINEDIDLMISRQRLCAVPPPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVSEDVDLLLNQRINNSQSAQSLATPVVSVATPTLPGQGMGGYPSAISTTYGTEYSLSSADLSSLSGFNTASALHLGSVTGWQQQHLHNMPPSALSQLGACTSTHLSQSSNLSLPSTQSLNIKSEPVSPPRDRTTTPSRYPQHTRHEAGRSPVDSLSSCSSSYDGSDREDHRNEFHSPIGLTRPSPDERESPSVKRMRLSEGWAT |
| 分子量 | 52.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MEF2C蛋白的3篇模拟参考文献及其摘要概述：
1. **文献名称**: *"Expression and Purification of Recombinant Human MEF2C Transcription Factor in Escherichia coli"*
**作者**: Potthoff, M.J. et al.
**摘要**: 该研究描述了在大肠杆菌系统中高效表达并纯化重组人MEF2C蛋白的方法，通过亲和层析获得高纯度蛋白，并验证了其体外结合DNA启动子区域的活性，为后续功能研究奠定基础。
2. **文献名称**: *"MEF2C Regulates Cardiac Morphogenesis via Transcriptional Control of Myocyte-Specific Genes"*
**作者**: Molkentin, J.D. & Olson, E.N.
**摘要**: 文章利用重组人MEF2C蛋白研究其在心脏发育中的转录调控作用，发现MEF2C通过激活心肌细胞特异性基因(如肌球蛋白重链)参与心脏形态发生，为先天性心脏病机制提供分子层面的解释。
3. **文献名称**: *"MEF2C Mediates Neurogenic Differentiation of Human Pluripotent Stem Cells"*
**作者**: Li, H. et al.
**摘要**: 研究通过体外递送重组人MEF2C蛋白至人诱导多能干细胞，证实其通过激活神经发育相关通路(如BDNF)促进神经元分化，为神经退行性疾病的细胞治疗提供潜在策略。
4. **文献名称**: *"Structural and Functional Analysis of MEF2C Mutations in Autism Spectrum Disorders"*
**作者**: Han, S. et al.
**摘要**: 该研究解析了自闭症相关MEF2C突变体重组蛋白的结构，发现关键结构域突变(如DNA结合区)导致转录活性受损，揭示了MEF2C功能异常与神经发育障碍的关联。
(注：以上文献为示例性模拟，实际引用请根据真实发表文献调整。)
Recombinant human MEF2C (myocyte enhancer factor 2C) protein is a engineered version of the transcription factor encoded by the MEF2C gene, which plays pivotal roles in cellular differentiation, development, and tissue-specific gene regulation. Belonging to the MEF2 family, MEF2C binds DNA through its MADS-box and MEF2 domains, regulating target genes involved in cardiac morphogenesis, neurogenesis, and skeletal muscle development. It is particularly critical during embryogenesis, where it coordinates heart and brain formation, and its dysfunction has been linked to neurodevelopmental disorders, intellectual disabilities, and cardiovascular diseases.
Recombinant MEF2C is typically produced using heterologous expression systems like E. coli or mammalian cells, followed by purification via affinity tags (e.g., His-tag). This allows researchers to study its biochemical properties, DNA-binding kinetics, and interactions with co-regulators (e.g., histone deacetylases) in vitro. Studies also employ recombinant MEF2C to explore its role in cellular reprogramming, neuronal plasticity, or disease mechanisms, such as its reduced activity in Alzheimer’s disease models. Additionally, it serves as a tool for screening small molecules aiming to modulate MEF2C-dependent pathways for therapeutic intervention. Its availability accelerates structural studies and functional assays, bridging gaps between genetic observations and mechanistic insights in developmental biology and disease.
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