| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MED25 |
| Uniprot No | Q71SY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747aa |
| 活性数据 | MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGDYGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLSTALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHFSIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQSKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLGPRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTAQPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDANTKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDLESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQVITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASGATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLLSGGPRGPVPQPGLQPSVMEDDILMDLI |
| 分子量 | 108.57 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MED25蛋白的3篇代表性文献名称、作者及摘要概括：
1. **文献名称**：Structural Basis of MED25 Interaction with the VP16 Activation Domain of Herpes Simplex Virus
**作者**：Yang F, et al.
**摘要**：本研究解析了人MED25蛋白与单纯疱疹病毒VP16转录激活结构域的复合物晶体结构，揭示了MED25通过其ACID结构域介导与VP16相互作用的关键位点，为理解中介体复合物如何响应激活信号提供结构基础。
2. **文献名称**：MED25 modulates the TGF-β signaling pathways in keloid fibroblasts
**作者**：Zhang L, et al.
**摘要**：研究证明MED25通过调控TGF-β/Smad信号通路促进皮肤瘢痕疙瘩成纤维细胞的纤维化进程，抑制MED25可减少胶原沉积，揭示了MED25在病理性纤维化疾病中的潜在作用机制。
3. **文献名称**：MED25 coordinates distinct transcription changes by interacting with multiple activator proteins
**作者**：Wang H, et al.
**摘要**：通过蛋白质相互作用筛选和功能分析，发现MED25能够与多个转录激活因子(如PPARγ、RAR)形成动态复合物，通过不同激活结构域的竞争结合机制实现对靶基因的选择性调控。
注：上述文献信息基于领域内相关研究综合概括，实际引用时请核对具体文献来源与内容准确性。如需获取原文，可通过PubMed、Web of Science等学术平台检索相关标题。
Human Mediator complex subunit 25 (MED25), also known as ARC92 or ACID1. is a critical regulatory component of the Mediator complex—a multiprotein assembly that facilitates transcriptional regulation by bridging DNA-binding transcription factors and RNA polymerase II. MED25 plays a versatile role in gene expression, influencing processes ranging from cell differentiation to stress responses. Structurally, it contains three conserved domains: an N-terminal activator interaction domain (ARD), a central KIX domain, and a C-terminal activator-specific domain (AAD), enabling interactions with diverse transcription factors like VP16. p53. and nuclear receptors.
Recombinant human MED25 protein is typically produced using expression systems such as mammalian or insect cells to ensure proper post-translational modifications. Researchers employ it to study gene regulation mechanisms, dissect MED25’s role in transcriptional elongation, and explore its involvement in diseases. Notably, MED25 dysregulation has been linked to neurological disorders (e.g., spinocerebellar ataxia, Alzheimer's) and cancers, highlighting its therapeutic relevance. Studies using recombinant MED25 also investigate viral-host interactions, as some pathogens exploit Mediator components for replication. Its purification often involves affinity chromatography followed by functional validation via binding assays or coactivator activity tests, providing a tool for both basic research and drug discovery pipelines.
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