| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCTP1 |
| Uniprot No | Q6DN14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-600aa |
| 活性数据 | MLDSCKLKSACNLPFICNKKIINTAGTSNAEVPLADPGMYQLDITLRRGQSLAARDRGGTSDPYVKFKIGGKEVFRSKIIHKNLNPVWEEKACILVDHLREPLYIKVFDYDFGLQDDFMGSAFLDLTQLELNRPTDVTLTLKDPHYPDHDLGIILLSVILTPKEGESRDVTMLMRKSWKRSSKELSENEVVGSYFSVKSLFWRTCGRPALPVLGFCRAELQNPYCKNVQFQTQSLRLSDLHRKSHLWRGIVSITLIEGRDLKAMDSNGLSDPYVKFRLGHQKYKSKIMPKTLNPQWREQFDFHLYEERGGVIDITAWDKDAGKRDDFIGRCQVDLSALSREQTHKLELQLEEGEGHLVLLVTLTASATVSISDLSVNSLEDQKEREEILKKYSPLRIFHNLKDVGFLQVKVIRAEGLMAADVTGKSDPFCVVELNNDRLLTHTVYKNLNPEWNKVFTFNIKDIHSVLEVTVYDEDRDRSADFLGKVAIPLLSIQNGEQKAYVLKNKQLTGPTKGVIYLEIDVIFNAVKASLRTLIPKEQKYIEEENRLSKQLLLRNFIRMKRCVMVLVNAAYYVNSCFDWDSPPRSLAAFVALYGTFINV |
| 分子量 | 91.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MCTP1蛋白的虚构参考文献示例(仅作格式参考，非真实文献)：
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1. **文献名称**: *Expression and Purification of Recombinant Human MCTP1 in Escherichia coli*
**作者**: Zhang Y. et al.
**摘要**: 该研究成功构建了人MCTP1蛋白的重组表达载体，利用大肠杆菌系统实现高效表达，并通过镍柱亲和层析纯化获得高纯度蛋白。作者验证了重组蛋白的C2结构域完整性，并探讨了其钙离子结合特性。
2. **文献名称**: *MCTP1 Regulates Neuronal Calcium Signaling via Interaction with Synaptotagmin*
**作者**: Lee S.J. et al.
**摘要**: 本文通过体外实验表明，重组人MCTP1蛋白能够与突触结合蛋白(synaptotagmin)发生特异性互作，调节神经突触的钙依赖性囊泡释放过程，揭示了MCTP1在神经元信号传导中的潜在作用。
3. **文献名称**: *Structural Analysis of Human MCTP1 Using Cryo-EM*
**作者**: Gupta R. et al.
**摘要**: 研究团队利用冷冻电镜技术解析了重组人MCTP1蛋白的跨膜区三维结构，发现其C末端螺旋对膜锚定至关重要，并提出了MCTP1可能通过多结构域协作参与细胞内膜运输的分子机制。
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如需真实文献，建议通过以下途径检索：
- **PubMed**：https://pubmed.ncbi.nlm.nih.gov/
- **Google Scholar**：https://scholar.google.com/
使用关键词：`"recombinant MCTP1"`, `"human MCTP1 protein"`, `"MCTP1 structure/function"`。
Recombinant human MCTP1 (Multiple C2 and Transmembrane Domain Protein 1) is a calcium-binding protein encoded by the *MCTP1* gene, belonging to the MCTP family characterized by unique structural features, including two C2 domains and transmembrane regions. The C2 domains mediate calcium-dependent phospholipid binding, suggesting MCTP1's involvement in calcium signaling and membrane trafficking processes. Its transmembrane domains anchor the protein to cellular membranes, potentially localizing it to organelles like the endoplasmic reticulum (ER) or Golgi apparatus.
MCTP1 has been implicated in diverse cellular functions, including vesicle transport, cell adhesion, and cytoskeletal organization. Studies suggest it may regulate synaptic vesicle exocytosis in neurons or secretory pathways in non-neuronal cells, linking calcium dynamics to membrane remodeling. Dysregulation of MCTP1 has been associated with pathological conditions, such as cancer progression (e.g., promoting tumor invasion or chemoresistance) and neurodevelopmental disorders. However, its precise molecular mechanisms remain incompletely understood.
Recombinant MCTP1 protein, typically produced in *E. coli* or mammalian expression systems with tags (e.g., His-tag), enables in vitro studies to dissect its biochemical interactions, calcium-binding properties, and structural-functional relationships. It serves as a critical tool for exploring MCTP1's role in cellular physiology and disease pathways, offering potential insights for therapeutic targeting.
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