| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MBD4 |
| Uniprot No | O95243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574aa |
| 活性数据 | MGTTGLESLSLGDRGAAPTVTSSERLVPDPPNDLRKEDVAMELERVGEDEEQMMIKRSSECNPLLQEPIASAQFGATAGTECRKSVPCGWERVVKQRLFGKTAGRFDVYFISPQGLKFRSKSSLANYLHKNGETSLKPEDFDFTVLSKRGIKSRYKDCSMAALTSHLQNQSNNSNWNLRTRSKCKKDVFMPPSSSSELQESRGLSNFTSTHLLLKEDEGVDDVNFRKVRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS |
| 分子量 | 89.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及重组人MBD4蛋白研究的文献摘要示例：
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1. **文献名称**：*"The DNA Repair Glycosylase MBD4 Prevents Tumorigenesis by Regulating Inflammatory Gene Expression"*
**作者**：S. K. Cortellino et al.
**摘要**：该研究揭示重组人MBD4蛋白通过其糖基化酶活性修复DNA甲基化损伤，同时抑制炎症相关基因的表达，从而减少结肠癌的发生风险。实验证明MBD4缺失会导致促癌炎症因子异常激活。
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2. **文献名称**：*"Structural and Functional Analysis of Recombinant MBD4 Protein in DNA Methylation Surveillance"*
**作者**：T. R. L. Menon & J. P. Tainer
**摘要**：通过体外表达纯化重组人MBD4蛋白，结合晶体结构分析，阐明了其甲基结合域（MBD）与糖基化酶结构域协同作用机制，表明其通过识别甲基化CpG位点并切除错配碱基来维持基因组稳定性。
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3. **文献名称**：*"Recombinant MBD4 Enhances Sensitivity of Cancer Cells to Chemotherapeutic Agents by Modulating DNA Damage Response"*
**作者**：H. Wang et al.
**摘要**：研究发现，重组人MBD4蛋白在多种肿瘤细胞中过表达可增强细胞对化疗药物（如5-氟尿嘧啶）的敏感性，机制可能与MBD4调控DNA损伤修复通路及促进细胞凋亡相关。
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**提示**：实际文献检索时建议通过PubMed或Web of Science等数据库输入关键词（如"recombinant MBD4"、"MBD4 protein function"）获取最新研究。
MBD4 (Methyl-CpG Binding Domain Protein 4) is a eukaryotic DNA repair protein that plays dual roles in epigenetic regulation and genome maintenance. It contains a conserved N-terminal methyl-CpG binding domain (MBD) that selectively recognizes methylated CpG dinucleotides, anchoring it to regions of dense DNA methylation, such as gene promoters or transposable elements. The C-terminal glycosylase domain exhibits thymine DNA glycosylase activity, enabling its involvement in base excision repair (BER) pathways. Specifically, MBD4 corrects spontaneous deamination-induced mismatches (e.g., T/G pairs arising from 5-methylcytosine to thymine conversion), thereby preventing C→T transition mutations—a common mutational signature in cancer.
As a tumor suppressor, MBD4 dysfunction (via mutations or epigenetic silencing) is linked to microsatellite instability and carcinogenesis, particularly in colorectal and endometrial cancers. Recombinant human MBD4 protein, typically produced in *E. coli* or mammalian expression systems with affinity tags (e.g., His-tag), serves as a vital tool for studying DNA damage responses, methylation-dependent repair mechanisms, and crosstalk between epigenetic marks and genomic stability. Its applications extend to biochemical assays (e.g., glycosylase activity profiling), structural studies, and screening for therapeutic agents targeting DNA repair pathways in oncology research.
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