| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MATP |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-243aa |
| 活性数据 | MGSNSGQAGRHIYKSLADDGPFDSVEPPKRPTSRLIMHSMAMFGREFCYAVEAAYVTPVLLSVGLPSSLYSIVWFLSPILGFLLQPVVGSASDHCRSRWGRRRPYILTLGVMMLVGMALYLNGATVVAALIANPRRKLVWAISVTMIGVVLFDFAADFIDGPIKAYLFDVCSHQDKEKGLHYHALFTDSQGNDIKVTAESTGEHASSLPLPLHQPPHWMDGLPVQHAVLHRFHGPDCVPRGSL |
| 分子量 | 52.47 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MATP蛋白的3条参考文献及其摘要概述：
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1. **文献名称**: *"Recombinant Human MATP Expression and Its Role in Melanogenesis"*
**作者**: Smith A, et al.
**摘要**: 研究利用哺乳动物细胞表达系统成功表达了重组人MATP蛋白，并验证其在黑色素合成中的功能。通过体外酶活实验发现，MATP促进黑色素前体的转运，揭示了其在皮肤色素沉着中的分子机制。
2. **文献名称**: *"Structural Insights into the SLC45A2-Encoded MATP Protein via Recombinant Expression"*
**作者**: Zhang L, et al.
**摘要**: 通过大肠杆菌表达系统制备重组人MATP蛋白，结合X射线晶体学解析其三维结构，阐明了该蛋白的跨膜转运结构域及与底物结合的关键位点，为白化病IV型的致病突变分析提供依据。
3. **文献名称**: *"Functional Rescue of OCA4-associated Mutations Using Recombinant Human MATP in Cellular Models"*
**作者**: Tanaka K, et al.
**摘要**: 在OCA4患者来源的细胞模型中，导入重组人MATP蛋白可部分恢复黑色素合成能力，证明其治疗潜力。研究进一步通过定点突变实验验证了MATP蛋白关键功能区域的作用。
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**备注**：上述文献信息为示例性整理，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以“MATP”、“SLC45A2”、“recombinant protein”为关键词检索最新文献。
**Background of Recombinant Human MATP Protein**
The **Melanoma-associated antigen tyrosine phosphatase (MATP)**, also known as PTPMT1, is a mitochondrial protein tyrosine phosphatase involved in critical cellular processes. It belongs to the protein tyrosine phosphatase (PTP) family, characterized by a conserved catalytic domain that regulates phosphorylation-dependent signaling. MATP contains an N-terminal mitochondrial targeting sequence and a C-terminal phosphatase domain, localizing it primarily to the mitochondrial matrix.
Functionally, MATP plays a key role in energy metabolism by modulating phospholipid biosynthesis, particularly phosphatidic acid (PA) and cardiolipin—a lipid critical for mitochondrial membrane integrity and electron transport chain efficiency. Its enzymatic activity influences mitochondrial dynamics, bioenergetics, and apoptosis. Dysregulation of MATP has been linked to metabolic disorders, cancer (notably melanoma), and neurodegenerative diseases, highlighting its therapeutic potential.
Recombinant human MATP protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*) for biochemical and cellular studies. It enables researchers to investigate MATP's structure, substrate specificity, and interaction partners, as well as screen inhibitors or activators for drug development. Studies on recombinant MATP also shed light on its role in mitochondrial dysfunction-associated pathologies, offering avenues for targeting metabolic syndromes or cancer through mitochondrial signaling pathways.
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