| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MASTL |
| Uniprot No | Q96GX5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-879aa |
| 活性数据 | MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQVLSLISSLGFNTPIAEKNQDPANILSACLSETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPGMPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSSPKWEKDCQESDEALGPTMMSWNAVEKLCAKSANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWEAVELDVNNINMDTDTSQLGFHQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQDLKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDRASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQESNQKMLGPPLEVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQKRRSCMPHQQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDWENLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL |
| 分子量 | 122.43 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional characterization of human MASTL kinase domain"**
- **作者**: D. Rodriguez et al.
- **摘要**: 本研究解析了重组人MASTL激酶域的晶体结构，揭示了其与底物结合的活性位点特征，并验证了其通过磷酸化抑制PP2A磷酸酶的机制，为靶向MASTL的抑制剂设计提供结构基础。
2. **"MASTL promotes mitotic progression by regulating PP2A-B55 dynamics in cancer cells"**
- **作者**: S. Kumar & L. H. Chen
- **摘要**: 研究通过CRISPR敲低和重组MASTL回补实验，证明MASTL通过调控PP2A-B55的亚细胞定位和活性，驱动有丝分裂完成，且在乳腺癌细胞中高表达MASTL与化疗抵抗相关。
3. **"High-yield expression and purification of recombinant human MASTL in E. coli for biochemical assays"**
- **作者**: M. Tanaka et al.
- **摘要**: 报道了一种基于大肠杆菌的高效重组人MASTL蛋白表达及纯化方案，通过优化密码子和纯化步骤获得高活性蛋白，成功用于体外激酶活性检测和抗体开发。
4. **"MASTL deficiency triggers genomic instability via aberrant Aurora B signaling"**
- **作者**: E. Park et al.
- **摘要**: 利用重组MASTL突变体，研究揭示了MASTL缺失导致Aurora B活性异常，引发染色体错分离和基因组不稳定性，提示其在结直肠癌中的抑癌作用。
（注：以上文献为虚构示例，实际文献需通过数据库查询。）
MASTL (microtubule-associated serine/threonine kinase-like protein), also known as Greatwall kinase, is a critical regulator of cell cycle progression, particularly during mitosis. It plays a pivotal role in maintaining mitotic fidelity by suppressing the activity of protein phosphatase 2A (PP2A), a key phosphatase that counteracts mitotic kinase signals. By phosphorylating endogenous inhibitors of PP2A, MASTL ensures proper mitotic entry, progression, and exit. Dysregulation of MASTL has been linked to chromosomal instability, defective DNA damage response, and diseases including cancer. Recombinant human MASTL protein, produced using heterologous expression systems (e.g., E. coli or mammalian cells), enables biochemical studies of its kinase activity, substrate interactions, and regulatory mechanisms. Researchers use it to investigate cell cycle control pathways, screen potential therapeutic inhibitors, and explore its role in tumorigenesis. Recent studies also highlight its involvement in cellular stress responses and potential as a therapeutic target. The recombinant form typically includes functional domains like the kinase domain and regulatory regions, often engineered with affinity tags for purification and detection. Current research focuses on deciphering its complex regulatory networks and context-dependent functions in health and disease.
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