| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MARK2 |
| Uniprot No | Q7KZI7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-755aa |
| 活性数据 | MIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL |
| 分子量 | 110.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MARK2蛋白的3篇代表性文献（内容基于公开研究概括，非真实文献，仅示例格式）：
1. **文献名称**：*Structural and Functional Analysis of Human MARK2 Kinase*
**作者**：Smith A. et al.
**摘要**：研究了重组人MARK2蛋白的晶体结构，揭示了其催化域与PAR1蛋白的相互作用机制，表明MARK2通过磷酸化微管相关蛋白调控细胞极性。
2. **文献名称**：*Recombinant MARK2 Expression in E. coli and Its Role in Tau Phosphorylation*
**作者**：Li Y. et al.
**摘要**：报道了在大肠杆菌中高效表达重组人MARK2蛋白的方法，验证其体外催化tau蛋白过度磷酸化的活性，提示与阿尔茨海默病病理相关性。
3. **文献名称**：*Regulation of Cell Cycle by MARK2 via Phosphorylation of MAP/microtubule Affinity-regulating Kinases*
**作者**：Wang H. et al.
**摘要**：证明重组MARK2通过磷酸化下游靶蛋白（如MARK家族成员）参与细胞周期调控，影响有丝分裂纺锤体形成和细胞分裂进程。
如需实际文献，建议通过PubMed或Google Scholar搜索以下关键词：
- "Recombinant human MARK2 purification"
- "MARK2 kinase Alzheimer’s disease"
- "MARK2 PAR-1 signaling"
Recombinant human MARK2 (MAP/microtubule affinity-regulating kinase 2), also known as PAR-1b, is a serine/threonine kinase critical for regulating microtubule dynamics and cell polarity. It belongs to the AMP-activated protein kinase (AMPK)-related kinase family and plays essential roles in neuronal development, cell cycle control, and synaptic plasticity. MARK2 phosphorylates microtubule-associated proteins, such as tau, destabilizing microtubules and influencing cytoskeletal organization. Dysregulation of MARK2 is implicated in neurodegenerative diseases, particularly Alzheimer’s disease, where hyperphosphorylated tau forms neurofibrillary tangles.
The recombinant form is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling studies on its structure-function relationships and interactions. Its N-terminal catalytic domain and C-terminal regulatory tail, which includes a kinase-associated domain (KA1), are key targets for mechanistic investigations. Recombinant MARK2 is indispensable for drug discovery efforts aimed at modulating kinase activity to treat tauopathies or cancers, where MARK2 overexpression correlates with poor prognosis.
Recent research focuses on its role in signaling pathways like Wnt/β-catenin and its crosstalk with metabolic regulators. However, challenges remain in understanding context-dependent substrate specificity and autoregulatory mechanisms. Recombinant protein tools facilitate biochemical assays, structural studies (e.g., X-ray crystallography), and high-throughput inhibitor screening, advancing both basic and translational studies.
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