| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MARK1 |
| Uniprot No | Q9P0L2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-795aa |
| 活性数据 | MSARTPLPTV NERDTENHTS VDGYTEPHIQ PTKSSSRQNI PRCRNSITSA TDEQPHIGNY RLQKTIGKGN FAKVKLARHV LTGREVAVKI IDKTQLNPTS LQKLFREVRI MKILNHPNIV KLFEVIETEK TLYLVMEYAS GGEVFDYLVA HGRMKEKEAR AKFRQIVSAV QYCHQKYIVH RDLKAENLLL DGDMNIKIAD FGFSNEFTVG NKLDTFCGSP PYAAPELFQG KKYDGPEVDV WSLGVILYTL VSGSLPFDGQ NLKELRERVL RGKYRIPFYM STDCENLLKK LLVLNPIKRG SLEQIMKDRW MNVGHEEEEL KPYTEPDPDF NDTKRIDIMV TMGFARDEIN DALINQKYDE VMATYILLGR KPPEFEGGES LSSGNLCQRS RPSSDLNNST LQSPAHLKVQ RSISANQKQR RFSDHAGPSI PPAVSYTKRP QANSVESEQK EEWDKDVARK LGSTTVGSKS EMTASPLVGP ERKKSSTIPS NNVYSGGSMA RRNTYVCERT TDRYVALQNG KDSSLTEMSV SSISSAGSSV ASAVPSARPR HQKSMSTSGH PIKVTLPTIK DGSEAYRPGT TQRVPAASPS AHSISTATPD RTRFPRGSSS RSTFHGEQLR ERRSVAYNGP PASPSHETGA FAHARRGTST GIISKITSKF VRRDPSEGEA SGRTDTSRST SGEPKERDKE EGKDSKPRSL RFTWSMKTTS SMDPNDMMRE IRKVLDANNC DYEQKERFLL FCVHGDARQD SLVQWEMEVC KLPRLSLNGV RFKRISGTSI AFKNIASKIA NELKL |
| 分子量 | 89 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人MARK1蛋白的文献示例（部分为模拟内容，实际引用需核实）：
1. **文献名称**：Structure and function of human MARK1 kinase: Implications for Alzheimer's disease
**作者**：Sörensen CS, et al.
**摘要**：该研究解析了重组人MARK1蛋白的晶体结构，揭示了其ATP结合域的关键构象，并通过体外实验证明其在调节微管相关蛋白磷酸化中的活性，提示其与神经退行性疾病的相关性。
2. **文献名称**：Expression and purification of active human MARK1 in Escherichia coli for biochemical characterization
**作者**：Trinczek B, et al.
**摘要**：文章报道了利用大肠杆菌系统高效表达可溶性的重组人MARK1蛋白，并通过镍柱亲和层析纯化获得高纯度蛋白，验证了其激酶活性及对底物Tau蛋白的磷酸化能力。
3. **文献名称**：MARK1 regulates neuronal polarization via microtubule dynamics
**作者**：Drewes G, et al.
**摘要**：研究利用重组人MARK1蛋白进行体外细胞实验，发现其通过磷酸化微管相关蛋白调控神经元轴突形成，揭示了MARK1在细胞极性建立中的分子机制。
4. **文献名称**：A genome-wide siRNA screen identifies MARK1 as a modulator of Aβ toxicity
**作者**：Marx A, et al.
**摘要**：通过全基因组筛选，发现MARK1活性影响β-淀粉样蛋白（Aβ）的毒性，重组MARK1蛋白的功能实验表明其可能成为阿尔茨海默病治疗的潜在靶点。
（注：部分文献标题及作者为领域相关研究的代表性示例，实际引用时建议通过PubMed或Google Scholar核对具体文献。）
**Background of Recombinant Human MARK1 Protein**
MARK1 (Microtubule Affinity-Regulating Kinase 1), also known as PAR1, belongs to the AMPK-related serine/threonine kinase family. It plays a pivotal role in regulating microtubule dynamics and cell polarity by phosphorylating microtubule-associated proteins (e.g., Tau, MAP2/4), promoting their dissociation from microtubules. This activity is critical for processes like neuronal development, cell division, and intracellular transport. Dysregulation of MARK1 has been linked to neurological disorders, including Alzheimer’s disease (via Tau hyperphosphorylation) and cancer (through disrupted cell polarity and signaling pathways).
Recombinant human MARK1 protein is engineered *in vitro* using expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form. It typically includes kinase domains and functional motifs, enabling researchers to study its enzymatic activity, substrate interactions, and regulatory mechanisms in controlled settings. Its applications span *in vitro* kinase assays, drug discovery (e.g., inhibitors for neurodegenerative diseases), and structural studies to elucidate binding interfaces or mutation effects.
As a tool, recombinant MARK1 bridges mechanistic insights into cellular architecture and disease pathways, offering potential therapeutic targets. Ongoing research focuses on its crosstalk with signaling networks (e.g., Wnt, AMPK) and its role in brain development, highlighting its broad relevance in basic and translational biology.
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