| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP4K1 |
| Uniprot No | Q92918 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-833aa |
| 活性数据 | MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL |
| 分子量 | 118.58 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP4K1蛋白的3篇参考文献示例（注：部分文献信息为模拟概括，实际引用请根据真实文献调整）：
1. **文献名称**：**"Recombinant human MAP4K1 regulates T-cell signaling through JNK activation"**
**作者**：Li, X. et al.
**摘要**：该研究利用大肠杆菌表达系统成功制备了重组人MAP4K1蛋白，并验证其激酶活性。结果表明，MAP4K1通过磷酸化下游底物激活JNK信号通路，在T细胞受体（TCR）介导的炎症反应中起关键调控作用。
2. **文献名称**：**"Structural characterization of MAP4K1 and its interaction with inhibitors"**
**作者**：Wang, Y. et al.
**摘要**：作者通过X射线晶体学解析了重组人MAP4K1蛋白的催化结构域三维结构，并筛选出小分子抑制剂。研究发现，MAP4K1的ATP结合口袋具有独特的疏水区域，为开发自身免疫性疾病靶向药物提供了结构基础。
3. **文献名称**：**"MAP4K1 promotes tumor metastasis via ERK pathway in colorectal cancer"**
**作者**：Chen, H. et al.
**摘要**：研究利用重组人MAP4K1蛋白进行体外功能实验，发现其过表达通过激活ERK信号通路增强结直肠癌细胞的迁移和侵袭能力，提示MAP4K1可能成为癌症治疗的潜在靶点。
（注意：以上文献为模拟生成，实际研究中请通过PubMed、Web of Science等平台用关键词“MAP4K1”、“recombinant protein”或“HPK1”检索最新文献。）
Recombinant human MAP4K1 protein is a genetically engineered form of the mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), also known as hematopoietic progenitor kinase 1 (HPK1). MAP4K1 is a member of the Ste20 serine/threonine kinase family and functions as an upstream activator of the MAPK signaling pathway, which regulates cellular responses to stress, immune activation, and inflammatory processes. It plays a critical role in T-cell receptor signaling, apoptosis, and immune regulation by interacting with adaptor proteins like TRAF2 and modulating downstream pathways such as JNK and NF-κB. Dysregulation of MAP4K1 has been implicated in autoimmune diseases, cancer progression, and metabolic disorders, making it a potential therapeutic target.
The recombinant protein is typically produced in heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to ensure high purity and biological activity for research applications. Its production enables studies on MAP4K1's structure-function relationships, kinase activity, and interactions with signaling partners. Researchers use recombinant MAP4K1 to screen inhibitors, dissect immune signaling networks, and explore its role in pathologies. Its recombinant form bypasses challenges in isolating the native protein from tissues, offering scalability and consistency for drug discovery and mechanistic studies.
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