| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAL2 |
| Uniprot No | Q969L2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-176aa |
| 活性数据 | MSAGGASVPPPPNPAVSFPPPRVTLPAGPDILRTYSGAFVCLEILFGGLVWILVASSNVPLPLLQGWVMFVSVTAFFFSLLFLGMFLSGMVAQIDANWNFLDFAYHFTVFVFYFGAFLLEAAATSLHDLHCNTTITGQPLLSDNQYNINVAASIFAFMTTACYGCSLGLALRRWRP |
| 分子量 | 19.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAL2蛋白的模拟参考文献示例(基于研究背景合理虚构，建议通过学术数据库核实真实文献)：
1. **文献名称**：Expression and Purification of Recombinant Human MAL2 in E. coli for Structural Analysis
**作者**：Li et al.
**摘要**：本研究成功构建了人MAL2蛋白的重组表达系统，通过大肠杆菌表达并优化纯化条件，获得高纯度蛋白，为后续结构解析和功能研究奠定基础。
2. **文献名称**：MAL2 as a Potential Biomarker in Ovarian Cancer: Detection Using Recombinant Protein-based ELISA
**作者**：Wang et al.
**摘要**：通过重组人MAL2蛋白制备多克隆抗体，开发特异性ELISA检测方法，发现卵巢癌患者血清中MAL2水平显著升高，提示其作为诊断标记物的潜力。
3. **文献名称**：Role of Recombinant MAL2 in Epithelial-Mesenchymal Transition of Breast Cancer Cells
**作者**：Zhang & Gomez
**摘要**：体外实验证实重组MAL2蛋白可通过调控E-cadherin/β-catenin通路促进乳腺癌细胞侵袭转移，揭示其在肿瘤进展中的分子机制。
4. **文献名称**：Structural Characterization of MAL2 Tetraspan Domain by Cryo-EM
**作者**：Chen et al.
**摘要**：利用冷冻电镜解析重组人MAL2蛋白的膜结合结构域三维构象，阐明其与脂筏微域互作的关键氨基酸位点及其跨膜转运功能。
**注意**：以上文献为基于MAL2蛋白研究方向的模拟示例，实际文献请通过PubMed/Google Scholar搜索关键词(如 "recombinant human MAL2 protein"、"MAL2 biomarker")获取。
**Background of Recombinant Human MAL2 Protein**
MAL2 (mal, T-cell differentiation protein 2) is a member of the MAL proteolipid family, which is involved in membrane trafficking and lipid raft organization. The human *MAL2* gene encodes a small, tetraspanning membrane protein predominantly expressed in polarized epithelial cells. It plays a critical role in transcytosis, facilitating cargo transport from the Golgi to the plasma membrane, particularly in absorptive and secretory tissues. Dysregulation of MAL2 has been implicated in cancer progression, including breast, ovarian, and colorectal carcinomas, where its overexpression correlates with enhanced cell proliferation, invasion, and poor prognosis.
Recombinant MAL2 protein is engineered using prokaryotic or eukaryotic expression systems, enabling studies on its structure, function, and interaction networks. Its production allows researchers to investigate mechanisms underlying MAL2-associated pathologies, screen for therapeutic agents, and develop diagnostic tools. Research on recombinant MAL2 also contributes to understanding intracellular trafficking pathways and their roles in cellular homeostasis or disease, highlighting its potential as a biomarker or therapeutic target in oncology and other fields.
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