| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAK |
| Uniprot No | P20794 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-623aa |
| 活性数据 | MNRYTTMRQL GDGTYGSVLM GKSNESGELV AIKRMKRKFY SWDECMNLRE VKSLKKLNHA NVIKLKEVIR ENDHLYFIFE YMKENLYQLM KDRNKLFPES VIRNIMYQIL QGLAFIHKHG FFHRDMKPEN LLCMGPELVK IADFGLAREL RSQPPYTDYV STRWYRAPEV LLRSSVYSSP IDVWAVGSIM AELYMLRPLF PGTSEVDEIF KICQVLGTPK KSDWPEGYQL ASSMNFRFPQ CVPINLKTLI PNASNEAIQL MTEMLNWDPK KRPTASQALK HPYFQVGQVL GPSSNHLESK QSLNKQLQPL ESKPSLVEVE PKPLPDIIDQ VVGQPQPKTS QQPLQPIQPP QNLSVQQPPK QQSQEKPPQT LFPSIVKNMP TKPNGTLSHK SGRRRWGQTI FKSGDSWEEL EDYDFGASHS KKPSMGVFKE KRKKDSPFRL PEPVPSGSNH STGENKSLPA VTSLKSDSEL STAPTSKQYY LKQSRYLPGV NPKKVSLIAS GKEINPHTWS NQLFPKSLGP VGAELAFKRS NAGNLGSYAT YNQSGYIPSF LKKEVQSAGQ RIHLAPLNAT ASEYTWNTKT GRGQFSGRTY NPTAKNLNIV NRAQPIPSVH GRTDWVAKYG GHR |
| 分子量 | 70.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人MAK蛋白的参考文献简介，按期刊格式整理：
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1. **文献名称**: *Crystal structure of human MAK kinase reveals an atypical activation loop conformation*
**作者**: Yamamoto, K., et al.
**摘要**: 通过X射线晶体学解析了重组人MAK激酶的结构，揭示其活化环构象与典型激酶不同，暗示其可能通过非经典磷酸化途径调控功能，为靶向药物设计提供结构基础。
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2. **文献名称**: *MAK regulates ciliary length via LRRC23 phosphorylation in human cells*
**作者**: Wang, L., Smith, E.F.
**摘要**: 研究证明重组人MAK激酶通过磷酸化LRRC23蛋白调控纤毛长度，实验结合基因敲除和体外激酶活性检测，提出MAK在纤毛相关疾病中的潜在作用机制。
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3. **文献名称**: *High-yield expression and purification of recombinant human MAK protein in E. coli*
**作者**: González, R., et al.
**摘要**: 开发了一种利用大肠杆菌表达系统高效制备重组人MAK蛋白的方法，优化纯化步骤后获得高活性蛋白，为后续生化和药物筛选研究提供可靠材料来源。
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*注：以上文献名为示例参考，实际应用中需根据真实发表论文标题调整。建议通过PubMed/Google Scholar检索关键词“recombinant human MAK protein”获取具体文献。*
Recombinant human MAK (Male Germ Cell-Associated Kinase) protein is a engineered form of the serine/threonine kinase encoded by the *MAK* gene. MAK is primarily expressed in male germ cells and plays roles in spermatogenesis, cell cycle regulation, and cilia-related functions. Structurally, it contains an SH3 domain, a catalytic kinase domain, and a unique C-terminal domain involved in subcellular localization. Its dysfunction has been linked to retinal degeneration, infertility, and cancers. The association of *MAK* mutations with retinal ciliopathies, particularly retinitis pigmentosa, highlights its importance in photoreceptor maintenance. In cancer contexts, MAK dysregulation correlates with tumor progression in prostate and liver cancers. Recombinant MAK is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling exploration of its kinase activity, protein interactions, and signaling pathways. This tool aids in dissecting MAK's role in ciliogenesis, cell proliferation, and apoptosis, as well as screening potential therapeutics targeting MAK-associated diseases. Its applications extend to disease modeling, biomarker research, and elucidating molecular mechanisms underlying infertility and degenerative disorders.
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