| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAK3 |
| Uniprot No | Q9GZZ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-169aa |
| 活性数据 | MKGSRIELGDVTPHNIKQLKRLNQVIFPVSYNDKFYKDVLEVGELAKLAYFNDIAVGAVCCRVDHSQNQKRLYIMTLGCLAPYRRLGIGTKMLNHVLNICEKDGTFDNIYLHVQISNESAIDFYRKFGFEIIETKKNYYKRIEPADAHVLQKNLKVPSGQNADVQKTDN |
| 分子量 | 46.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAK3(或MARK3)蛋白的参考文献列举(经虚拟整合，非真实文献)：
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1. **标题**：*Expression and Purification of Recombinant Human MARK3 in Escherichia coli for Structural Studies*
**作者**：Chen L, et al.
**摘要**：报道了在大肠杆菌中高效表达可溶性重组人MARK3激酶结构域的方法，通过优化诱导条件和亲和层析纯化，获得高纯度蛋白，用于X射线晶体学研究。
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2. **标题**：*Functional Characterization of Recombinant MARK3 in Tau Protein Phosphorylation*
**作者**：Smith J, et al.
**摘要**：利用杆状病毒-昆虫细胞系统表达全长人MARK3.验证其激酶活性及对Tau蛋白的特异性磷酸化作用，揭示其在阿尔茨海默病相关信号通路中的潜在作用。
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3. **标题**：*Development of a Kinase Assay for High-Throughput Screening Using Recombinant Human MAK3*
**作者**：Gupta R, et al.
**摘要**：建立了基于重组人MAK3蛋白的体外激酶活性检测平台，用于筛选小分子抑制剂，为癌症和神经退行性疾病药物开发提供工具。
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4. **标题**：*Role of Recombinant MARK3 in Cell Polarity Regulation*
**作者**：Watanabe K, et al.
**摘要**：通过哺乳动物细胞表达系统研究重组MARK3对上皮细胞极性的调控，证明其通过磷酸化PAR1调控细胞骨架重排的机制。
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**注**：上述文献为示例性质，实际研究中建议通过PubMed或Web of Science以“MARK3”(Microtubule Affinity-Regulating Kinase 3)或“MAK3”为关键词检索最新论文，结合具体研究需求筛选。
Recombinant human MAK3 protein, also known as male germ cell-associated kinase 3. is a member of the protein kinase superfamily implicated in cellular signaling and post-translational regulation. MAK3 is encoded by the *MAK3* gene and shares homology with serine/threonine kinases involved in cell cycle control, apoptosis, and stress responses. Structurally, it contains a conserved kinase domain responsible for ATP binding and catalytic activity.
Originally identified in male germ cells, MAK3 is now recognized for broader roles in somatic tissues, including modulation of transcriptional activity and interaction with signaling pathways like MAPK/ERK. Dysregulation of MAK3 has been linked to diseases such as cancer, where it may influence tumor progression, and neurodegenerative disorders, potentially affecting protein aggregation. Its role in RNA binding and processing further suggests involvement in post-transcriptional regulation.
Recombinant MAK3 is typically produced using expression systems (e.g., E. coli, mammalian cells) to ensure proper folding and phosphorylation capacity. Purified recombinant protein enables in vitro studies to dissect kinase-substrate relationships, screen inhibitors, or analyze structural features via crystallography. As a research tool, it aids in exploring MAK3's physiological functions and validating its potential as a therapeutic target. Ongoing research focuses on clarifying its substrate specificity, regulatory networks, and disease-related mechanisms to inform drug development or diagnostic strategies.
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