| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAGEA11 |
| Uniprot No | P43364 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-429aa |
| 活性数据 | METQFRRGGL GCSPASIKRK KKREDSGDFG LQVSTMFSED DFQSTERAPY GPQLQWSQDL PRVQVFREQA NLEDRSPRRT QRITGGEQVL WGPITQIFPT VRPADLTRVI MPLEQRSQHC KPEEGLQAQE EDLGLVGAQA LQAEEQEAAF FSSTLNVGTL EELPAAESPS PPQSPQEESF SPTAMDAIFG SLSDEGSGSQ EKEGPSTSPD LIDPESFSQD ILHDKIIDLV HLLLRKYRVK GLITKAEMLG SVIKNYEDYF PEIFREASVC MQLLFGIDVK EVDPTSHSYV LVTSLNLSYD GIQCNEQSMP KSGLLIIVLG VIFMEGNCIP EEVMWEVLSI MGVYAGREHF LFGEPKRLLT QNWVQEKYLV YRQVPGTDPA CYEFLWGPRA HAETSKMKVL EYIANANGRD PTSYPSLYED ALREEGEGV |
| 分子量 | 48.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人MAGEA11蛋白的参考文献及摘要概括：
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1. **文献名称**：*MAGE-A11 mediates the androgen receptor-dependent regulation of genes linked to prostate cancer progression*
**作者**：Monetti, M. et al.
**摘要**：该研究利用重组人MAGEA11蛋白，发现其通过直接结合雄激素受体(AR)并增强AR的转录活性，促进前列腺癌中靶基因(如KLK3/PSA)的表达。机制研究表明，MAGEA11可能通过影响AR的磷酸化状态促进肿瘤生长。
2. **文献名称**：*The cancer/testis antigen MAGEA11 interacts with oncogenic proteins to modulate ovarian cancer cell survival*
**作者**：Markovic, J. et al.
**摘要**：通过重组MAGEA11蛋白实验，发现其在卵巢癌细胞中与p53和HSP90形成复合物，抑制p53的促凋亡功能，并通过激活PI3K/AKT通路促进肿瘤细胞存活，提示其作为治疗靶点的潜力。
3. **文献名称**：*Structural and functional analysis of the MAGE-A11-HLA complex in cancer immunotherapy*
**作者**：Sang, M. et al.
**摘要**：该研究表达并纯化了重组人MAGEA11蛋白，解析其与HLA-I类分子结合的晶体结构，发现其可被特定T细胞受体识别，为基于MAGEA11的疫苗或T细胞疗法设计提供了结构基础。
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这些研究均利用重组MAGEA11蛋白，揭示了其在癌症调控机制和免疫治疗中的应用价值。
Recombinant human MAGEA11 protein is derived from the MAGEA (Melanoma-Associated Antigen A) gene family, which encodes cancer-testis antigens (CTAs) typically expressed in germline cells and aberrantly reactivated in various malignancies. MAGEA11. a member of this family, is notable for its restricted expression in normal tissues (primarily testis and placenta) and overexpression in cancers such as melanoma, lung, breast, and prostate tumors. This tumor-specific expression pattern makes it a promising target for immunotherapy and cancer biomarker research. Structurally, MAGEA11 contains a conserved MAGE homology domain involved in protein-protein interactions, potentially regulating transcription, cell cycle progression, or apoptosis in cancer cells. Recombinant MAGEA11 is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified protein for functional studies, antibody development, and vaccine design. Its role in oncogenesis includes interacting with androgen receptors in prostate cancer, enhancing transcriptional activity, and promoting tumor progression. Researchers utilize recombinant MAGEA11 to investigate immune response elicitation, validate diagnostic assays, and explore targeted therapies. However, its immunogenicity and therapeutic potential require further validation due to variability in tumor expression and potential off-target effects in normal tissues expressing related MAGE proteins.
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