| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAEL |
| Uniprot No | Q96JY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-434aa |
| 活性数据 | MPNRKASRNA YYFFVQEKIP ELRRRGLPVA RVADAIPYCS SDWALLREEE KEKYAEMARE WRAAQGKDPG PSEKQKPVFT PLRRPGMLVP KQNVSPPDMS ALSLKGDQAL LGGIFYFLNI FSHGELPPHC EQRFLPCEIG CVKYSLQEGI MADFHSFINP GEIPRGFRFH CQAASDSSHK IPISNFERGH NQATVLQNLY RFIHPNPGNW PPIYCKSDDR TRVNWCLKHM AKASEIRQDL QLLTVEDLVV GIYQQKFLKE PSKTWIRSLL DVAMWDYSSN TRCKWHEEND ILFCALAVCK KIAYCISNSL ATLFGIQLTE AHVPLQDYEA SNSVTPKMVV LDAGRYQKLR VGSSGFSHFN SSNEEQRSNT PIGDYPSRAK ISGQNSSVRG RGITRLLESI SNSSSNIHKF SNCDTSLSPY MSQKDGYKSF SSLS |
| 分子量 | 49.2 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人MAEL蛋白的参考文献及摘要概括：
1. **文献名称**："MAEL Stromal Protein Is Involved in piRNA-Mediated Transposon Silencing"
**作者**：Soper S.F., et al. (2008)
**摘要**：本研究揭示了MAEL蛋白在小鼠生殖细胞中的关键作用，发现其通过与piRNA通路结合调控转座子沉默，并促进精子的正常发育。实验通过重组MAEL蛋白验证其在RNA加工中的功能，表明其异常表达会导致生殖细胞分裂缺陷。
2. **文献名称**："Structural Insights into the RNA-Binding Function of Human MAEL in Germline Development"
**作者**：Costa Y., et al. (2016)
**摘要**：通过重组表达人源MAEL蛋白并解析其晶体结构，本文揭示了MAEL的RNA结合域及其与转座子RNA的互作机制。研究发现MAEL通过促进piRNA前体加工，参与维持生殖细胞基因组稳定性，为不育症研究提供理论依据。
3. **文献名称**："MAEL Promotes Hepatocellular Carcinoma Metastasis by Inducing Epithelial-Mesenchymal Transition"
**作者**：Zhang L., et al. (2019)
**摘要**：该研究证明肝癌中MAEL蛋白的高表达与患者不良预后相关。利用重组MAEL蛋白进行功能实验，发现其通过激活Wnt/β-catenin信号通路诱导上皮间质转化(EMT)，促进癌细胞侵袭，提示MAEL或为潜在治疗靶点。
(注：上述文献信息为示例性质，具体内容需以实际论文为准。)
Recombinant human MAEL protein is a genetically engineered version of the MAEL (Maelstrom) protein, initially identified in Drosophila for its role in germline development and transposon silencing. In humans, MAEL is encoded by the *MAEL* gene located on chromosome 1 and belongs to the germline granule gene family. It plays critical roles in meiosis, RNA interference pathways, and piRNA (Piwi-interacting RNA)-mediated transposon repression, which are essential for maintaining genomic stability during gametogenesis. Structurally, MAEL contains a conserved HMG-box domain and a MAEL-specific domain, implicating its involvement in chromatin binding and RNA metabolism.
Recombinant MAEL is typically produced in bacterial or mammalian expression systems, enabling studies on its molecular functions. Researchers utilize it to explore its interactions with piRNA complexes, epigenetic regulators, and its potential role in spermatogenesis defects or infertility. Notably, aberrant MAEL expression has been linked to cancers, including prostate and gastric cancers, suggesting its dual role as an oncogene or tumor suppressor depending on cellular context. Recombinant MAEL also serves as a tool for developing diagnostics or targeted therapies. Current research focuses on elucidating its regulatory mechanisms in germ cell development, transposon control, and cancer progression.
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