| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAD1L1 |
| Uniprot No | Q9Y6D9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-718aa |
| 活性数据 | MEDLGENTMV LSTLRSLNNF ISQRVEGGSG LDISTSAPGS LQMQYQQSMQ LEERAEQIRS KSHLIQVERE KMQMELSHKR ARVELERAAS TSARNYEREV DRNQELLTRI RQLQEREAGA EEKMQEQLER NRQCQQNLDA ASKRLREKED SLAQAGETIN ALKGRISELQ WSVMDQEMRV KRLESEKQEL QEQLDLQHKK CQEANQKIQE LQASQEARAD HEQQIKDLEQ KLSLQEQDAA IVKNMKSELV RLPRLERELK QLREESAHLR EMRETNGLLQ EELEGLQRKL GRQEKMQETL VGLELENERL LAKLQSWERL DQTMGLSIRT PEDLSRFVVE LQQRELALKD KNSAVTSSAR GLEKARQQLQ EELRQVSGQL LEERKKRETH EALARRLQKR VLLLTKERDG MRAILGSYDS ELTPAEYSPQ LTRRMREAED MVQKVHSHSA EMEAQLSQAL EELGGQKQRA DMLEMELKML KSQSSSAEQS FLFSREEADT LRLKVEELEG ERSRLEEEKR MLEAQLERRA LQGDYDQSRT KVLHMSLNPT SVARQRLRED HSQLQAECER LRGLLRAMER GGTVPADLEA AAASLPSSKE VAELKKQVES AELKNQRLKE VFQTKIQEFR KACYTLTGYQ IDITTENQYR LTSLYAEHPG DCLIFKATSP SGSKMQLLET EFSHTVGELI EVHLRRQDSI PAFLSSLTLE LFSRQTVA |
| 分子量 | 83 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于常见研究主题模拟的关于重组人MAD1L1蛋白的参考文献示例(实际文献需通过学术数据库获取)：
1. **名称**: "Expression and functional characterization of recombinant human MAD1L1 in mitotic checkpoint control"
**作者**: Zhang Y, et al.
**摘要**: 研究报道了通过大肠杆菌系统表达并纯化重组人MAD1L1蛋白，验证其与MAD2L1的相互作用，证明其维持有丝分裂检查点功能的必要性。
2. **名称**: "Structural insights into MAD1L1 protein dynamics during spindle assembly"
**作者**: Lee S, et al.
**摘要**: 利用重组MAD1L1蛋白进行X射线晶体学研究，揭示了其N端结构域在微管结合中的构象变化，及其对染色体排列的调控机制。
3. **名称**: "MAD1L1 mutations disrupt cellular tension sensing in cancer cells"
**作者**: Gupta R, et al.
**摘要**: 通过体外重组蛋白实验，证明MAD1L1突变体导致细胞对纺锤体张力信号响应异常，与结直肠癌基因组不稳定性相关。
4. **名称**: "In vivo role of MAD1L1 in transgenic mouse models"
**作者**: Watanabe K, et al.
**摘要**: 构建条件性敲除MAD1L1的小鼠模型，结合重组蛋白回补实验，发现其缺失引发胚胎致死性染色体错配，强调发育中的必需性。
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**说明**：上述内容为模拟方向，真实文献建议通过PubMed、Web of Science等平台以“recombinant MAD1L1”或“MAD1L1 protein function”等关键词检索。研究多集中于该蛋白的有丝分裂检查点调控、癌症关联突变及结构生物学分析。
MAD1L1 (Mitotic Arrest Deficient 1 Like 1) is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures faithful chromosome segregation during mitosis. It acts as a scaffold protein localized to unattached kinetochores, where it recruits and activates other SAC proteins, including MAD2L1. to inhibit the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes achieve proper bipolar attachment. This checkpoint prevents aneuploidy, a hallmark of cancer and genetic disorders. Dysregulation of MAD1L1 has been linked to tumorigenesis, chromosomal instability, and chemoresistance.
Recombinant human MAD1L1 protein is engineered for functional and structural studies to dissect its role in SAC signaling. Produced via heterologous expression systems (e.g., bacteria, insect, or mammalian cells), it retains key functional domains such as the kinetochore-binding region and MAD2-interaction motifs. Researchers utilize this protein to investigate kinetochore-microtubule dynamics, SAC activation mechanisms, and its interplay with cancer-associated mutations. Its recombinant form also facilitates high-throughput drug screening for SAC-targeted therapies and serves as an antigen for antibody development. Structural analyses (e.g., X-ray crystallography) employing purified MAD1L1 have revealed conformational changes critical for SAC propagation. However, challenges persist in producing full-length MAD1L1 due to its large size (>200 kDa) and intrinsic disorder, often prompting studies on truncated variants. Current research focuses on leveraging recombinant MAD1L1 to elucidate its non-mitotic roles in cellular stress responses and interphase functions.
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