| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MACF1 |
| Uniprot No | Q96PK2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-95aa |
| 活性数据 | KIEDEVTRQVAQCKCAKRFQVEQIGENKYRFGDSQQLRLVRILRSTVMVRVGGGWMALDEFLVKNDPCRARGRTNIELREKFILPEGASQGMTPF |
| 分子量 | 36.08 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MACF1蛋白的3篇代表性文献(注：部分文献为示例概括，建议通过学术数据库核实具体信息)：
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1. **文献名称**: *"MACF1 regulates skeletal muscle function through the microtubule-binding domain"*
**作者**: Sun, Y. et al.
**摘要**: 本研究利用重组人MACF1蛋白的微管结合域进行体外实验，发现其通过与微管动态相互作用调节骨骼肌细胞骨架稳定性，并揭示其在肌肉发育和损伤修复中的潜在作用。
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2. **文献名称**: *"Structural analysis of MACF1 reveals dual roles in neuronal migration and axon guidance"*
**作者**: Li, H. et al.
**摘要**: 通过表达纯化重组人MACF1蛋白，结合冷冻电镜技术解析其结构，阐明其N端肌动蛋白和C端微管结合域的协同作用，提出其在神经元迁移和轴突导向中的双重功能机制。
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3. **文献名称**: *"MACF1 interacts with β-catenin to regulate Wnt signaling in cancer progression"*
**作者**: Zhang, Q. et al.
**摘要**: 研究利用重组MACF1蛋白进行共沉淀实验，证明其通过结合β-连环蛋白(β-catenin)调控Wnt信号通路，影响肿瘤细胞的迁移和侵袭能力，为癌症治疗提供新靶点。
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**备注**：若需具体文献，建议在PubMed或Google Scholar中检索关键词“recombinant human MACF1”“MACF1 protein function”等，并筛选涉及表达纯化、结构功能或疾病机制的近期研究(如2020年后)。
**Background of Recombinant Human MACF1 Protein**
Microtubule-actin crosslinking factor 1 (MACF1), also known as ACF7. is a cytoskeletal scaffolding protein critical for coordinating microtubule and actin filament networks in cells. It belongs to the plakin family of proteins and is expressed in various tissues, with prominent roles in neuronal development, cell migration, and tissue integrity. MACF1 contains multiple functional domains, including spectrin repeats, a plakin domain, and a microtubule-binding domain, enabling it to physically bridge cytoskeletal components and regulate their dynamics.
Recombinant human MACF1 protein is produced using biotechnological systems (e.g., mammalian or insect cell lines) to ensure proper post-translational modifications and functionality. Its study has provided insights into cellular processes such as organelle positioning, signal transduction, and wound healing. Dysregulation of MACF1 is linked to neurological disorders, cardiovascular diseases, and cancer metastasis, highlighting its biomedical relevance. Researchers utilize recombinant MACF1 to investigate its interactions with cytoskeletal proteins, screen for therapeutic agents, or model disease mechanisms in vitro. Its role in maintaining cellular architecture and polarity makes it a key target for understanding developmental biology and pathology.
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