| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAC30 |
| Uniprot No | Q5BJF2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-69aa |
| 活性数据 | MTTLIPILSTFLFEDFSKASGFKGQRPETLHERLTLVSVYAPYLLIPFILLIFMLRSPYYKYEEKRKKK |
| 分子量 | 34.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAC30蛋白的3条模拟参考文献及摘要内容：
1. **文献名称**: "Cloning and high-yield expression of recombinant human MAC30 in Escherichia coli"
**作者**: Zhang L. et al.
**摘要**: 该研究报道了通过大肠杆菌表达系统高效克隆与表达重组人MAC30蛋白的方法，优化了诱导条件与纯化步骤，获得高纯度蛋白用于后续功能研究。
2. **文献名称**: "Structural analysis of recombinant human MAC30 reveals a conserved glioma-associated antigen motif"
**作者**: Wang Y. et al.
**摘要**: 利用X射线晶体学解析了重组人MAC30的蛋白结构，发现其含有与胶质瘤抗原相关的特征性结构域，为研究其在肿瘤中的分子机制提供依据。
3. **文献名称**: "Recombinant MAC30 protein modulates TGF-β signaling in pancreatic cancer cells"
**作者**: Kim S. & Patel R.
**摘要**: 研究表明重组MAC30蛋白通过调控TGF-β通路抑制胰腺癌细胞侵袭和迁移，提示其作为潜在治疗靶点的可能性。
(注：以上文献信息为示例模拟，实际引用时需以真实出版物为准。)
Recombinant human MAC30 protein, also known as transmembrane protein 97 (TMEM97), is a conserved eukaryotic protein linked to diverse cellular processes. First identified as overexpressed in meningiomas, MAC30 has since been associated with cancer progression, cholesterol homeostasis, and neurodegenerative disorders. It localizes to intracellular membranes, particularly the trans-Golgi network and lysosomes, interacting with proteins like NPC1 and EGFR to influence signaling pathways. Structurally, it contains a conserved UPF0005 domain, though its exact biological role remains unclear.
Recombinant MAC30 is typically produced in *E. coli* or mammalian systems for biochemical studies. Its production enables research into its proposed dual functionality: as a potential tumor biomarker promoting cell proliferation/metastasis in cancers (e.g., gliomas, pancreatic cancer), and as a modulator of cholesterol trafficking with implications for Alzheimer’s disease pathology. Emerging evidence suggests involvement in σ-2 receptor signaling, linking it to cellular stress responses and drug targeting. Current applications span structural analysis, interaction mapping, and therapeutic development, particularly for cancers and neurodegenerative conditions. However, mechanistic details of its physiological functions require further elucidation.
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