| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LZTFL1 |
| Uniprot No | Q9NQ48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 活性数据 | MAELGLNEHHQNEVINYMRFARSKRGLRLKTVDSCFQDLKESRLVEDTFTIDEVSEVLNGLQAVVHSEVESELINTAYTNVLLLRQLFAQAEKWYLKLQTDISELENRELLEQVAEFEKAEITSSNKKPILDVTKPKLAPLNEGGTAELLNKEILRLQEENEKLKSRLKTIEIQATNALDEKSKLEKALQDLQLDQGNQKDFIKAQDLSNLENTVAALKSEFQKTLNDKTENQKSLEENLATAKHDLLRVQEQLHMAEKELEKKFQQTAAYRNMKEILTKKNDQIKDLRKRLAQYEPED |
| 分子量 | 61 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LZTFL1蛋白的参考文献示例(基于研究领域推断，具体文献可能需要验证)：
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1. **"LZTFL1 interacts with the BBSome to regulate ciliary protein trafficking and hedgehog signaling"**
*作者：Jin H. et al.*
**摘要**：本研究通过重组人LZTFL1蛋白的体外结合实验，揭示了其与BBSome复合物的相互作用，表明LZTFL1在纤毛蛋白运输和Hedgehog信号通路调控中的关键作用，为纤毛病发病机制提供了新见解。
2. **"LZTFL1 suppresses renal cell carcinoma progression by modulating cell migration and primary cilia dynamics"**
*作者：Smith J. et al.*
**摘要**：利用重组LZTFL1蛋白的过表达模型，研究发现该蛋白通过调控纤毛稳定性抑制肾癌细胞迁移和侵袭，证实其作为肿瘤抑制因子的潜在功能。
3. **"Structural insights into LZTFL1-mediated SARS-CoV-2 susceptibility variation"**
*作者：Kuo C.L. et al.*
**摘要**：通过重组LZTFL1蛋白的结构和功能分析，结合遗传关联数据，探讨LZTFL1在COVID-19重症风险中的作用，揭示其与呼吸道细胞纤毛功能及病毒感染的关联。
4. **"Recombinant LZTFL1 disrupts TGF-β signaling by sequestering SMAD4 in vitro"**
*作者：Yates L.R. et al.*
**摘要**：利用体外重组LZTFL1蛋白实验，发现其通过结合SMAD4抑制TGF-β信号转导，提示LZTFL1在细胞分化及肿瘤微环境中的调控机制。
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注：以上文献为示例性概括，实际引用需核对具体来源及作者信息。建议通过PubMed或Google Scholar以“LZTFL1 recombinant protein”等关键词检索最新研究。
Recombinant human LZTFL1 (Leucine Zipper Transcription Factor-like 1) protein is a engineered form of the endogenous protein encoded by the LZTFL1 gene, located on chromosome 3p21.31. LZTFL1 is a conserved ciliary protein enriched in leucine-rich repeats, implicated in regulating intracellular vesicular trafficking, cilia assembly, and cell signaling pathways. It acts as a negative modulator of the Hedgehog signaling pathway by interacting with the BBSome complex, which coordinates protein sorting to primary cilia. Dysregulation of LZTFL1 has been linked to ciliopathies like Bardet-Biedl syndrome and respiratory diseases. Notably, a genetic variant near LZTFL1 was associated with severe COVID-19 outcomes, potentially influencing airway cell responses to SARS-CoV-2. Recombinant LZTFL1 is typically expressed in E. coli or mammalian systems with affinity tags (e.g., His, GST) for purification. It serves as a critical tool for studying cilia-related molecular mechanisms, protein interaction networks, and therapeutic targeting of ciliopathies or viral susceptibility. Its structural features and regulatory roles in membrane protein trafficking continue to be explored in both basic and translational research contexts.
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