| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LYK5 |
| Uniprot No | Q7RTN6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-431aa |
| 活性数据 | MSFLVSKPER IRRWVSEKFI VEGLRDLELF GEQPPGDTRR KTNDASSESI ASFSKQEVMS SFLPEGGCYE LLTVIGKGFE DLMTVNLARY KPTGEYVTVR RINLEACSNE MVTFLQGELH VSKLFNHPNI VPYRATFIAD NELWVVTSFM AYGSAKDLIC THFMDGMNEL AIAYILQGVL KALDYIHHMG YVHRSVKASH ILISVDGKVY LSGLRSNLSM ISHGQRQRVV HDFPKYSVKV LPWLSPEVLQ QNLQGYDAKS DIYSVGITAC ELANGHVPFK DMPATQMLLE KLNGTVPCLL DTSTIPAEEL TMSPSRSVAN SGLSDSLTTS TPRPSNGDSP SHPYHRTFSP HFHHFVEQCL QRNPDARPSA STLLNHSFFK QIKRRASEAL PELLRPVTPI TNFEGSQSQD HSGIFGLVTN LEELEVDDWE F |
| 分子量 | 48.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LYK5蛋白的示例参考文献(注：LYK5蛋白名称及文献内容为假设性示例，仅供参考)：
1. **文献名称**：**"Expression and Functional Characterization of Recombinant Human LYK5 in Mammalian Cells"**
**作者**：Smith A. et al.
**摘要**：本研究通过哺乳动物表达系统成功表达并纯化了重组人LYK5蛋白，验证了其与配体结合的活性，揭示了其在细胞信号转导中的潜在作用。
2. **文献名称**：**"Structural Analysis of LYK5 Reveals a Unique Kinase Domain Architecture"**
**作者**：Zhang L. et al.
**摘要**：通过X射线晶体学解析了重组人LYK5蛋白的晶体结构，发现其激酶结构域具有独特的折叠模式，为靶向药物设计提供了结构基础。
3. **文献名称**：**"LYK5 as a Novel Therapeutic Target in Triple-Negative Breast Cancer"**
**作者**：Jones R. et al.
**摘要**：研究表明，重组人LYK5蛋白的过表达与乳腺癌细胞迁移相关，抑制LYK5活性可显著降低肿瘤侵袭性，提示其作为治疗靶点的潜力。
4. **文献名称**：**"Recombinant LYK5 Protein Modulates Immune Responses in Macrophages"**
**作者**：Chen Y. et al.
**摘要**：实验证实，重组LYK5蛋白通过调控NF-κB通路影响巨噬细胞的炎症因子分泌，揭示了其在免疫调节中的功能。
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**注意**：以上文献为假设性示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。若LYK5蛋白名称存在拼写或命名差异，建议结合基因编号(如UniProt)或功能关键词进一步确认。
Recombinant human LYK5 protein is a genetically engineered form of the lysine kinase LYK5 (also known as lymphocyte-specific kinase), which plays a regulatory role in intracellular signaling pathways. LYK5 belongs to the tyrosine kinase family and is implicated in modulating immune responses, cell proliferation, and differentiation. It is structurally characterized by conserved kinase domains and specific phosphorylation sites, enabling interactions with downstream signaling molecules like STAT proteins. Dysregulation of LYK5 has been linked to autoimmune disorders and cancers, highlighting its potential as a therapeutic target.
The recombinant protein is typically produced using expression systems such as E. coli or mammalian cells, ensuring post-translational modifications critical for functional activity. Purification methods like affinity chromatography yield high-purity LYK5. which is utilized in biochemical assays, drug screening, and mechanistic studies to elucidate its role in disease pathways. Researchers also employ it to develop kinase inhibitors or antibodies for diagnostic and therapeutic applications. Its recombinant form provides a controlled, scalable source for consistent experimental outcomes, advancing both basic research and translational efforts in oncology and immunology. Continued studies on LYK5 aim to clarify its signaling network and therapeutic potential in precision medicine.
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