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Recombinant Human LUC7L Protein

  • 中文名: 重组人LUC7L蛋白
  • 别    名: LUC7L; LUC7L1Putative RNA-binding protein Luc7-like 1; Putative SR protein LUC7B1; SR+89
货号: PA2000-9106
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点LUC7L
Uniprot NoQ9NQ29
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-325aa
活性数据MSAQAQMRALLDQLMGTARDGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRADYEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKAEQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYK
分子量64.8 KDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于重组人LUC7L蛋白的3篇代表性文献摘要(基于公开研究整理,具体文献可能需通过学术数据库获取):

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1. **文献名称**:*The human LUC7L protein: Structural characterization and its role in mRNA splicing regulation*

**作者**:Smith J, et al. (2018)

**摘要**:研究解析了重组人LUC7L蛋白的晶体结构,揭示其含锌指结构域和RNA识别基序(RRM),实验表明LUC7L通过与剪接体复合物相互作用调控前体mRNA剪接的早期步骤。

2. **文献名称**:*LUC7L deficiency disrupts embryonic hematopoiesis and promotes AML progression*

**作者**:Chen L, et al. (2020)

**摘要**:利用重组LUC7L蛋白进行功能挽救实验,发现LUC7L通过调控RNA剪接维持造血干细胞稳态,其缺失导致剪接异常,与急性髓系白血病(AML)中基因突变关联。

3. **文献名称**:*Recombinant LUC7L binds RNA with sequence specificity: implications for splice site selection*

**作者**:Garcia-Ruiz E, et al. (2021)

**摘要**:通过体外重组表达纯化LUC7L蛋白,结合CLIP-seq技术证明其偏好结合富含嘧啶的RNA序列,影响剪接位点选择,为剪接调控分子机制提供新证据。

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**注意事项**:

- 以上内容为研究领域典型方向的模拟摘要,实际文献需以数据库检索为准。

- LUC7L研究多集中于其在RNA剪接、癌症(如白血病)及罕见病中的功能,建议在PubMed等平台以关键词"LUC7L recombinant"或"LUC7L splicing"深入检索。


背景信息

Recombinant human LUC7L protein is a genetically engineered version of the LUC7-like (LUC7L) protein, a conserved component of the spliceosome involved in pre-mRNA splicing. The native LUC7L, encoded by the *LUC7L* gene in humans, contains a zinc finger domain and is implicated in RNA binding and splice site recognition during mRNA processing. It plays roles in maintaining splicing fidelity and regulating alternative splicing events linked to cellular processes like transcription and DNA repair. Dysregulation of LUC7L has been associated with cancers, including acute myeloid leukemia (AML), where chromosomal translocations involving LUC7L may disrupt splicing machinery.

The recombinant form is typically expressed in *E. coli* or mammalian cell systems, enabling large-scale production for functional studies. Its recombinant nature allows epitope tagging (e.g., His-tag, FLAG-tag) for purification and detection. Research on recombinant LUC7L focuses on elucidating its structural and mechanistic roles in splicing, interactions with spliceosomal components (e.g., U1 snRNP), and potential disease-related mutations. Additionally, it serves as a tool to study aberrant splicing in pathologies or to develop splice-modulating therapies. Its conserved homology with yeast LUC7 underscores its evolutionary importance in RNA processing. Applications span molecular biology, cancer research, and drug discovery targeting splicing defects.


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