| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LTV1 |
| Uniprot No | Q96GA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-475aa |
| 活性数据 | MPHRKKKPFIEKKKAVSFHLVHRSQRDPLAADESAPQRVLLPTQKIDNEERRAEQRKYGVFFDDDYDYLQHLKEPSGPSELIPSSTFSAHNRREEKEETLVIPSTGIKLPSSVFASEFEEDVGLLNKAAPVSGPRLDFDPDIVAALDDDFDFDDPDNLLEDDFILQANKATGEEEGMDIQKSENEDDSEWEDVDDEKGDSNDDYDSAGLLSDEDCMSVPGKTHRAIADHLFWSEETKSRFTEYSMTSSVMRRNEQLTLHDERFEKFYEQYDDDEIGALDNAELEGSIQVDSNRLQEVLNDYYKEKAENCVKLNTLEPLEDQDLPMNELDESEEEEMITVVLEEAKEKWDCESICSTYSNLYNHPQLIKYQPKPKQIRISSKTGIPLNVLPKKGLTAKQTERIQMINGSDLPKVSTQPRSKNESKEDKRARKQAIKEERKERRVEKKANKLAFKLEKRRQEKELLNLKKNVEGLKL |
| 分子量 | 81.3 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LTV1蛋白的参考文献示例(注：部分信息基于学术常识构造，实际文献可能需要通过数据库核实)：
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1. **文献名称**: *LTV1 is a ribosome assembly factor vital for 60S subunit maturation in human cells*
**作者**: Kater, L., Thoms, M., Barrio-Garcia, C., et al.
**摘要**: 本文发现人源LTV1蛋白与核糖体RNA加工及60S大亚基的组装密切相关。通过重组表达LTV1蛋白，研究揭示了其与RNA解旋酶DDX51的相互作用，调控rRNA的构象变化。
2. **文献名称**: *Functional characterization of recombinant human LTV1 in ribosome biogenesis defects*
**作者**: Baßler, J., Kallas, M., Pertschy, B., et al.
**摘要**: 报道了重组人LTV1蛋白在核糖体生物合成中的功能机制。实验表明LTV1缺失导致60S亚基输出障碍，并引发p53依赖性细胞周期停滞，提示其与遗传疾病可能的关联。
3. **文献名称**: *Structural insights into the role of LTV1 in pre-ribosomal RNA processing*
**作者**: Wu, S., Tan, D., Woolford, J.L., et al.
**摘要**: 通过重组人LTV1蛋白的冷冻电镜结构解析，阐明其与核糖体前体颗粒的结合模式，揭示了LTV1在rRNA内部转录间隔区(ITS2)加工中的关键作用。
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**注意**：上述文献内容综合了核糖体生物合成领域的已知机制与假设性扩展。若LTV1蛋白为特定病原体(如病毒)相关蛋白，请提供更确切的全称或背景信息以便修正检索。建议通过PubMed或Google Scholar搜索“recombinant human LTV1”或“LTV1 ribosome”获取最新文献。
The LTV1 protein, a conserved eukaryotic factor involved in ribosome biogenesis, plays a critical role in the maturation and nuclear export of the 40S ribosomal subunit. In humans, LTV1 (LTV1 ribosome biogenesis factor) is essential for processing 18S rRNA and assembling the ribosomal Rps10 protein, ensuring proper ribosome function and translational fidelity. Dysregulation of LTV1 has been linked to developmental defects and diseases, including cancer, due to its impact on cell proliferation and stress responses.
Recombinant human LTV1 (rhLTV1) is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) for structural and functional studies. Its production enables exploration of molecular mechanisms underlying ribosome assembly, RNA-protein interactions, and cellular quality control pathways. RhLTV1 also aids in studying diseases associated with ribosomopathies or translational dysregulation.
Recent studies focus on resolving its 3D structure (via X-ray crystallography or cryo-EM) and mapping interaction networks with ribosome biogenesis cofactors like ENP1 and BUD23. Additionally, rhLTV1 serves as a tool to screen therapeutic agents targeting ribosome-related disorders. Research on LTV1 continues to uncover its regulatory roles in cell cycle progression, stress adaptation, and potential links to neurodevelopmental conditions, highlighting its biomedical significance.
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