| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LTK |
| Uniprot No | P29376 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-864aa |
| 活性数据 | MGCWGQLLVWFGAAGAILCSSPGSQETFLRSSPLPLASPSPRDPKVSAPPSILEPASPLNSPGTEGSWLFSTCGASGRHGPTQTQCDGAYAGTSVVVTVGAAGQLRGVQLWRVPGPGQYLISAYGAAGGKGAKNHLSRAHGVFVSAIFSLGLGESLYILVGQQGEDACPGGSPESQLVCLGESRAVEEHAAMDGSEGVPGSRRWAGGGGGGGGATYVFRVRAGELEPLLVAAGGGGRAYLRPRDRGRTQASPEKLENRSEAPGSGGRGGAAGGGGGWTSRAPSPQAGRSLQEGAEGGQGCSEAWATLGWAAAGGFGGGGGACTAGGGGGGYRGGDASETDNLWADGEDGVSFIHPSSELFLQPLAVTENHGEVEIRRHLNCSHCPLRDCQWQAELQLAECLCPEGMELAVDNVTCMDLHKPPGPLVLMVAVVATSTLSLLMVCGVLILVKQKKWQGLQEMRLPSPELELSKLRTSAIRTAPNPYYCQVGLGPAQSWPLPPGVTEVSPANVTLLRALGHGAFGEVYEGLVIGLPGDSSPLQVAIKTLPELCSPQDELDFLMEALIISKFRHQNIVRCVGLSLRATPRLILLELMSGGDMKSFLRHSRPHLGQPSPLVMRDLLQLAQDIAQGCHYLEENHFIHRDIAARNCLLSCAGPSRVAKIGDFGMARDIYRASYYRRGDRALLPVKWMPPEAFLEGIFTSKTDSWSFGVLLWEIFSLGYMPYPGRTNQEVLDFVVGGGRMDPPRGCPGPVYRIMTQCWQHEPELRPSFASILERLQYCTQDPDVLNSLLPMELGPTPEEEGTSGLGNRSLECLRPPQPQELSPEKLKSWGGSPLGPWLSSGLKPLKSRGLQPQNLWNPTYRS |
| 分子量 | 91.6 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LTK蛋白的3篇代表性文献的简要信息，供参考：
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1. **文献名称**：Structural basis of ALK/LTK activation by neurotrophic ligands
**作者**：Wang, Z., et al.
**摘要**：通过冷冻电镜解析了重组人LTK蛋白与其配体结合的结构，揭示LTK激酶结构域激活的分子机制，为靶向药物开发提供依据。
2. **文献名称**：Functional characterization of recombinant human LTK kinase domain in vitro
**作者**：Liu, Y., & Smith, J.
**摘要**：研究重组表达的LTK激酶结构域的生化特性，证实其体外自磷酸化活性及底物特异性，为激酶抑制剂筛选提供模型。
3. **文献名称**：LTK promotes cell proliferation via MAPK pathway in cancer models
**作者**：Chen, H., et al.
**摘要**：利用重组人LTK蛋白处理肿瘤细胞，验证其通过激活MAPK信号通路促进细胞增殖，提示LTK在癌症中的潜在作用。
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*注：LTK(Leukocyte Tyrosine Kinase)常与ALK同源，研究多集中于结构解析、激酶活性及其在疾病中的功能。以上文献信息为模拟示例，实际文献需通过PubMed或Sci-Hub等平台检索。建议用关键词"recombinant human LTK"或"LTK kinase domain"查找具体研究。*
Recombinant human LTK (Leukocyte Tyrosine Kinase) protein is a engineered form of the LTK receptor, a member of the receptor tyrosine kinase (RTK) family. LTK is primarily expressed in hematopoietic cells and shares structural homology with ALK (Anaplastic Lymphoma Kinase). It consists of an extracellular ligand-binding domain, a transmembrane region, and an intracellular tyrosine kinase domain. While LTK’s physiological ligand remains unclear, it plays roles in cell proliferation, differentiation, and survival, potentially through pathways like JAK-STAT or PI3K-AKT. Dysregulation of LTK signaling has been linked to cancers, particularly leukemia and lymphoma, where activating mutations may drive oncogenesis. However, LTK’s functional mechanisms are less studied compared to ALK, partly due to limited natural expression and undefined ligand interactions.
Recombinant LTK production, often using mammalian or insect cell systems, enables researchers to study its structure, signaling, and pathogenic mutations. It serves as a critical tool for screening kinase inhibitors, developing therapeutic antibodies, and understanding molecular mechanisms in hematologic malignancies. Recent interest in LTK stems from its potential as a druggable target, especially in ALK-negative cancers, highlighting the need for further exploration of its biological and therapeutic significance.
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