| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LTB4R |
| Uniprot No | Q15722 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-352aa |
| 活性数据 | MNTTSSAAPPSLGVEFISLLAIILLSVALAVGLPGNSFVVWSILKRMQKRSVTALMVLNLALADLAVLLTAPFFLHFLAQGTWSFGLAGCRLCHYVCGVSMYASVLLITAMSLDRSLAVARPFVSQKLRTKAMARRVLAGIWVLSFLLATPVLAYRTVVPWKTNMSLCFPRYPSEGHRAFHLIFEAVTGFLLPFLAVVASYSDIGRRLQARRFRRSRRTGRLVVLIILTFAAFWLPYHVVNLAEAGRALAGQAAGLGLVGKRLSLARNVLIALAFLSSSVNPVLYACAGGGLLRSAGVGFVAKLLEGTGSEASSTRRGGSLGQTARSGPAALEPGPSESLTASSPLKLNELN |
| 分子量 | 37.5 KDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LTB4R蛋白的参考文献及摘要概括(文献为示例性内容，可能与实际发表文献存在差异)：
1. **《Functional Characterization of the Human Leukotriene B4 Receptor Cloned in Mammalian Cells》**
*作者：Yokomizo T., Izumi T., Shimizu T. (1997)*
**摘要**：首次成功克隆了人源LTB4R基因，并在哺乳动物细胞中实现重组表达。研究发现重组受体能与LTB4高亲和力结合，介导细胞内钙离子信号传导，证实其在炎症反应中的关键作用。
2. **《Crystal Structure of the Human LTB4R Bound to Antagonist》**
*作者：Hori T., Okuno T., Hirata K. et al. (2018)*
**摘要**：通过X射线晶体学解析了重组人LTB4R蛋白与拮抗剂复合物的三维结构，揭示了配体结合口袋的关键氨基酸残基，为抗炎药物设计提供结构基础。
3. **《Recombinant LTB4R Expression in E. coli and Its Role in Neutrophil Migration》**
*作者：Chen L., Smith M.L., Hwang S.H. (2005)*
**摘要**：利用大肠杆菌系统高效表达重组LTB4R蛋白，功能实验表明该蛋白可通过激活MAPK通路调控中性粒细胞趋化运动，验证其在炎症性疾病中的功能。
4. **《Targeting Recombinant LTB4R in Murine Asthma Models》**
*作者：Kim D., Park J.Y., Lee H. (2020)*
**摘要**：在小鼠哮喘模型中研究重组LTB4R蛋白的作用，发现阻断LTB4R信号可显著减轻气道炎症和黏液分泌，提示其作为治疗靶点的潜力。
**Background of Recombinant Human LTB4R Protein**
Leukotriene B4 receptor (LTB4R), also known as BLT1. is a G protein-coupled receptor (GPCR) that binds leukotriene B4 (LTB4), a potent lipid mediator derived from arachidonic acid. LTB4R plays a critical role in inflammatory and immune responses by mediating chemotaxis, adhesion, and activation of leukocytes, particularly neutrophils. Its signaling is implicated in chronic inflammatory diseases, such as asthma, rheumatoid arthritis, and atherosclerosis, making it a target for therapeutic interventions.
Recombinant human LTB4R protein is engineered using expression systems like mammalian or insect cells to ensure proper post-translational modifications and functional activity. This protein retains the native receptor's structural and ligand-binding properties, enabling studies on LTB4-LTB4R interactions, receptor activation mechanisms, and downstream signaling pathways. It is widely utilized in drug discovery to screen antagonists or modulators that could attenuate harmful inflammatory responses. Additionally, recombinant LTB4R aids in structural biology research, such as crystallography or cryo-EM, to elucidate receptor-ligand binding interfaces and guide rational drug design. By providing a consistent, high-purity protein source, recombinant LTB4R accelerates both basic research and therapeutic development targeting inflammation-related disorders.
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