| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LTB4DH |
| Uniprot No | Q14914 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 活性数据 | MVRTKTWTLK KHFVGYPTNS DFELKTAELP PLKNGEVLLE ALFLTVDPYM RVAAKRLKEG DTMMGQQVAK VVESKNVALP KGTIVLASPG WTTHSISDGK DLEKLLTEWP DTIPLSLALG TVGMPGLTAY FGLLEICGVK GGETVMVNAA AGAVGSVVGQ IAKLKGCKVV GAVGSDEKVA YLQKLGFDVV FNYKTVESLE ETLKKASPDG YDCYFDNVGG EFSNTVIGQM KKFGRIAICG AISTYNRTGP LPPGPPPEIV IYQELRMEAF VVYRWQGDAR QKALKDLLKW VLEGKIQYKE YIIEGFENMP AAFMGMLKGD NLGKTIVKA |
| 分子量 | 35.8 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为重组人LTB4DH蛋白相关研究的简要参考文献列举：
1. **《Expression and characterization of recombinant human leukotriene B4 12-hydroxydehydrogenase》**
**作者**: Yokomizo T. et al.
**摘要**: 首次报道了利用大肠杆菌系统成功表达重组人LTB4DH蛋白，证实其催化LTB4转化为12-oxo-LTB4的活性，并分析了其酶动力学参数。
2. **《Structural insights into LTB4DH substrate specificity by crystallographic analysis》**
**作者**: Chen X. et al.
**摘要**: 通过X射线晶体学解析重组人LTB4DH的三维结构，揭示了其底物结合口袋的构象特征，解释了该酶对羟基类脂分子的催化特异性机制。
3. **《Functional role of LTB4DH in inflammatory resolution using knockout cell models》**
**作者**: Singh R.K. et al.
**摘要**: 通过在HEK293细胞中重组表达/敲除LTB4DH，证实该酶通过降解促炎介质LTB4.负向调控中性粒细胞介导的炎症反应通路。
4. **《Optimization of recombinant LTB4DH production in Pichia pastoris》**
**作者**: Wang H. et al.
**摘要**: 开发毕赤酵母高效分泌表达重组LTB4DH的工艺，通过启动子优化和糖基化修饰将酶活性提升3倍，为工业化生产奠定基础。
注：LTB4DH全称为Leukotriene B4 12-hydroxydehydrogenase(EC 1.1.1.210)，上述文献为模拟概括性内容，实际研究需通过PubMed或Web of Science等平台检索具体论文。
Leukotriene B4 dehydrogenase (LTB4DH), also known as 12-hydroxydehydrogenase, is an NAD+-dependent enzyme that plays a critical role in the inactivation of leukotriene B4 (LTB4), a potent pro-inflammatory lipid mediator. LTB4 is involved in neutrophil chemotaxis, immune responses, and chronic inflammatory diseases. LTB4DH catalyzes the oxidation of LTB4 to 12-oxo-LTB4. thereby dampening its pro-inflammatory effects. Dysregulation of LTB4 metabolism has been linked to pathologies like asthma, rheumatoid arthritis, and atherosclerosis. Recombinant human LTB4DH protein is typically produced using eukaryotic or bacterial expression systems, enabling studies on its enzymatic activity, substrate specificity, and interaction with inhibitors. Its structural and functional characterization aids in understanding inflammatory pathways and developing therapeutics targeting LTB4 signaling. Research applications include enzyme kinetics assays, drug screening, and cellular models of inflammation. The recombinant protein’s availability also supports antibody production and biomarker studies in inflammatory diseases. Recent interest in LTB4DH stems from its potential as a regulatory checkpoint in resolving inflammation, offering avenues for precision anti-inflammatory therapies. Current challenges involve optimizing its stability and catalytic efficiency for industrial-scale use.
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