| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LSM2 |
| Uniprot No | Q9Y333 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-95aa |
| 活性数据 | MLFYSFFKSLVGKDVVVELKNDLSICGTLHSVDQYLNIKLTDISVTDPEKYPHMLSVKNCFIRGSVVRYVQLPADEVDTQLLQDAARKEALQQKQ |
| 分子量 | 36.85 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人LSM2蛋白相关的参考文献(示例基于假设性研究，实际文献需通过学术数据库查询补充)：
---
1. **文献名称**: *Recombinant expression and functional characterization of human LSM2 in spliceosome assembly*
**作者**: Müller S, et al.
**摘要**: 报道了人源LSM2蛋白在大肠杆菌中的重组表达及纯化，分析其与U6 snRNA的结合能力，并验证其在体外剪接体组装中的关键作用。
---
2. **文献名称**: *Structural insights into LSM2-8 complex in RNA processing*
**作者**: Zhou Y, et al.
**摘要**: 通过X射线晶体学解析LSM2-8蛋白复合体的结构，阐明LSM2与其他亚基的相互作用，揭示其在pre-mRNA剪接中的分子机制。
---
3. **文献名称**: *LSM2 knockdown and overexpression alter mRNA stability in human cells*
**作者**: Gupta R, et al.
**摘要**: 研究利用重组LSM2蛋白进行细胞回补实验，证明LSM2通过结合特定RNA序列调控靶标mRNA的稳定性，影响细胞周期进程。
---
提示：实际文献建议在 **PubMed** 或 **Google Scholar** 中搜索关键词 "recombinant human LSM2"、"LSM2 protein function" 或 "spliceosome LSM2 complex"。知名期刊如 *Nature Structural & Molecular Biology* 或 *RNA* 可能有相关研究。
Recombinant human LSM2 protein is a genetically engineered form of the LSM2 (Like Sm 2) protein, a conserved component of eukaryotic RNA-processing complexes. LSM2 belongs to the Sm-like protein family, which plays critical roles in RNA metabolism, including pre-mRNA splicing, mRNA decay, and RNA quality control. In humans, LSM2 primarily associates with the nuclear LSM2-8 heteroheptameric complex, facilitating U6 small nuclear RNA (snRNA) stabilization and spliceosome assembly. It also participates in cytoplasmic mRNA degradation pathways via interactions with the LSM1-7 complex. Recombinant LSM2 is typically expressed in bacterial or eukaryotic systems to study its molecular functions, structural properties, and interactions with RNA or protein partners. Its production enables research into cellular mechanisms underlying RNA processing dysregulation linked to diseases such as cancer, neurodegeneration, and viral infections. By providing a purified, well-characterized tool, recombinant LSM2 aids in structural analyses (e.g., X-ray crystallography) and drug discovery targeting RNA-protein networks. Current studies also explore its role in stress granules and processing bodies, highlighting its broad relevance to post-transcriptional gene regulation.
×
