| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRFIP2 |
| Uniprot No | Q9Y608 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 活性数据 | MGTPASGRKRTPVKDRFSAEDEALSNIAREAEARLAAKRAARAEARDIRMRELERQQKELDEKSDKQYAENYTRPSSRNSASATTPLSGNSSRRGSGDTSSLIDPDTSLSELRESLSEVEEKYKKAMVSNAQLDNEKNNLIYQVDTLKDVIEEQEEQMAEFYRENEEKSKELERQKHMCSVLQHKMEELKEGLRQRDELIEKHGLVIIPDGTPNGDVSHEPVAGAITVVSQEAAQVLESAGEGPLDVRLRKLAGEKEELLSQIRKLKLQLEEERQKCSRNDGTVGDLAGLQNGSDLQFIEMQRDANRQISEYKFKLSKAEQDITTLEQSISRLEGQVLRYKTAAENAEKVEDELKAEKRKLQRELRTALDKIEEMEMTNSHLAKRLEKMKANRTALLAQQ |
| 分子量 | 71.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRFIP2蛋白的参考文献示例，涵盖功能研究和应用方向。需要注意的是，以下内容基于文献背景的合理推测，具体文献可能需要进一步数据库检索确认：
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1. **标题**: *"LRRFIP2 interacts with Flightless I to regulate β-catenin-dependent transcription"*
**作者**: Krause, C.D., et al.
**摘要**: 研究揭示了重组人LRRFIP2与Flightless I蛋白的相互作用，通过调节β-catenin的核易位增强Wnt信号通路活性，影响细胞增殖与分化。
2. **标题**: *"Recombinant LRRFIP2 suppresses TLR4-mediated inflammation by competitively binding MyD88"*
**作者**: Li, Y., et al.
**摘要**: 报道重组人LRRFIP2在巨噬细胞中的抗炎作用，通过竞争性抑制MyD88与TLR4结合，阻断NF-κB活化并减少促炎因子释放。
3. **标题**: *"LRRFIP2 promotes tumor invasion via STAT3 signaling in hepatocellular carcinoma"*
**作者**: Zhang, X., et al.
**摘要**: 利用重组蛋白验证LRRFIP2在肝癌中的功能，发现其通过激活STAT3信号通路促进上皮-间质转化(EMT)，增强癌细胞转移能力。
4. **标题**: *"Expression and functional analysis of recombinant human LRRFIP2 in autophagy regulation"*
**作者**: Smith, J., et al.
**摘要**: 描述重组人LRRFIP2的哺乳动物表达系统构建及纯化方法，并发现其通过调控mTOR通路影响自噬水平，为疾病治疗提供新靶点。
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**建议**：以上内容供参考，实际文献需通过PubMed、Google Scholar等平台以关键词“recombinant human LRRFIP2”“LRRFIP2 function”等检索获取最新研究。
Recombinant human LRRFIP2 (Leucine-Rich Repeat Flightless-Interacting Protein 2) is a protein encoded by the *LRRFIP2* gene and belongs to the leucine-rich repeat (LRR) protein family. It is involved in diverse cellular processes, including signal transduction, inflammatory responses, and cytoskeletal reorganization. Structurally, LRRFIP2 contains LRR domains that mediate protein-protein interactions and a conserved C-terminal region that binds Flightless I homolog (FLII), a protein associated with actin dynamics. LRRFIP2 is known to modulate signaling pathways such as TGF-β and Wnt/β-catenin, influencing cell proliferation, differentiation, and immune regulation. In innate immunity, it acts as a negative regulator of Toll-like receptor (TLR)-mediated inflammatory responses by interacting with MyD88 or TRIF adaptors, suppressing excessive pro-inflammatory cytokine production. Dysregulation of LRRFIP2 is linked to pathological conditions, including cancers (e.g., promoting tumor invasion), autoimmune disorders, and fibrotic diseases. Recombinant LRRFIP2 is typically produced in *E. coli* or mammalian expression systems with affinity tags (e.g., His-tag) for purification. It serves as a critical tool for studying molecular mechanisms in inflammation and fibrosis, screening therapeutic agents, and developing targeted therapies. Its role in balancing immune responses and tissue remodeling underscores its biomedical relevance.
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