| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC8E |
| Uniprot No | Q6NSJ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-796aa |
| 活性数据 | MIPVAEFKQFTEQQPAFKVLKPWWDVLAEYLTVAMLMIGVFGCTLQVTQDKIICLPNHELQENLSEAPCQQLLPRGIPEQIGALQEVKGLKNNLDLQQYSFINQLCYETALHWYTKYFPYLVVIHTLIFMVCTSFWFKFPGTSSKIEHFISILGKCFDSPWTTRALSEVSGENQKGPAATERAAATIVAMAGTGPGKAGEGEKEKVLAEPEKVVTEPPVVTLLDKKEGEQAKALFEKVKKFRMHVEEGDILYTMYIRQTVLKVCKFLAILVYNLVYVEKISFLVACRVETSEVTGYASFCCNHTKAHLFSKLAFCYISFVCIYGLTCIYTLYWLFHRPLKEYSFRSVREETGMGDIPDVKNDFAFMLHLIDQYDSLYSKRFAVFLSEVSESRLKQLNLNHEWTPEKLRQKLQRNAAGRLELALCMLPGLPDTVFELSEVESLRLEAICDITFPPGLSQLVHLQELSLLHSPARLPFSLQVFLRDHLKVMRVKCEELREVPLWVFGLRGLEELHLEGLFPQELARAATLESLRELKQLKVLSLRSNAGKVPASVTDVAGHLQRLSLHNDGARLVALNSLKKLAALRELELVACGLERIPHAVFSLGALQELDLKDNHLRSIEEILSFQHCRKLVTLRLWHNQIAYVPEHVRKLRSLEQLYLSYNKLETLPSQLGLCSGLRLLDVSHNGLHSLPPEVGLLQNLQHLALSYNALEALPEELFFCRKLRTLLLGDNQLSQLSPHVGALRALSRLELKGNRLEALPEELGNCGGLKKAGLLVEDTLYQGLPAEVRDKMEEE |
| 分子量 | 116.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人LRRC8E蛋白的3篇代表性文献摘要：
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1. **"Structure of the volume-regulated anion channel LRRC8 complex"**
*作者：Qiu et al. (2018), Nature*
摘要：通过冷冻电镜解析LRRC8异源复合物(含LRRC8A/E亚基)的结构，揭示其作为体积调节性阴离子通道(VRAC)的核心功能。研究发现LRRC8E可能参与调节通道的离子选择性及门控机制。
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2. **"LRRC8 proteins form heteromeric complexes to enable functional diversity in volume-regulated anion channels"**
*作者：Deneka et al. (2018), eLife*
摘要：系统分析不同LRRC8亚基(包括LRRC8E)组合对VRAC通道电导率及底物运输的影响，指出LRRC8E的参与可能增强通道对特定代谢物(如抗肿瘤药物顺铂)的转运效率。
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3. **"LRRC8E is essential for swelling-activated glutamate release via VRAC in cancer cells"**
*作者：Schober et al. (2021), Cell Reports*
摘要：证明LRRC8E在癌细胞中与LRRC8A形成复合物，介导细胞肿胀触发的谷氨酸释放，促进肿瘤微环境重塑及化疗耐药性，提示LRRC8E作为潜在抗癌靶点。
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**补充说明**：
LRRC8E属LRRC8家族成员，主要通过与核心亚基LRRC8A结合形成功能性阴离子通道，参与细胞体积调节、代谢物转运及疾病病理过程。相关研究多聚焦于其结构特征、亚基互作机制及在癌症中的功能。
The leucine-rich repeat-containing 8 (LRRC8) family, including LRRC8E, comprises transmembrane proteins that form volume-regulated anion channels (VRACs). These channels regulate cellular processes such as osmotic balance, metabolite transport, and cell signaling. LRRC8 proteins typically function as heteromers, with LRRC8A being an essential subunit for channel activity. LRRC8E, one of the five family members (LRRC8A-E), contributes to the structural and functional diversity of VRACs by forming heterohexameric complexes with LRRC8A. This combinatorial assembly influences ion selectivity, gating, and substrate specificity, enabling tailored responses to cell swelling or shrinkage. Recombinant human LRRC8E protein is produced using heterologous expression systems (e.g., HEK293 or insect cells) for structural and functional studies. It aids in elucidating VRAC architecture, ion transport mechanisms, and roles in pathologies like cancer, stroke, and immune regulation. Research on recombinant LRRC8E also supports drug discovery, as VRACs are therapeutic targets for modulating cell volume-related disorders. The protein’s structure includes a conserved leucine-rich repeat domain and variable N- and C-terminal regions that mediate subunit interactions and regulatory signals. Studies leverage techniques like cryo-EM, electrophysiology, and gene editing to decode its biological and clinical relevance.
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