| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC8B |
| Uniprot No | Q6P9F7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-803aa |
| 活性数据 | MITLTELKCLADAQSSYHILKPWWDVFWYYITLIMLLVAVLAGALQLTQSRVLCCLPCKVEFDNHCAVPWDILKASMNTSSNPGTPLPLPLRIQNDLHRQQYSYIDAVCYEKQLHWFAKFFPYLVLLHTLIFAACSNFWLHYPSTSSRLEHFVAILHKCFDSPWTTRALSETVAEQSVRPLKLSKSKILLSSSGCSADIDSGKQSLPYPQPGLESAGIESPTSSVLDKKEGEQAKAIFEKVKRFRMHVEQKDIIYRVYLKQIIVKVILFVLIITYVPYFLTHITLEIDCSVDVQAFTGYKRYQCVYSLAEIFKVLASFYVILVILYGLTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIFLSEVSENKLKQINLNNEWTVEKLKSKLVKNAQDKIELHLFMLNGLPDNVFELTEMEVLSLELIPEVKLPSAVSQLVNLKELRVYHSSLVVDHPALAFLEENLKILRLKFTEMGKIPRWVFHLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLSLDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLRENNLKTVEEIISFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLDHNNIENLPLQLFLCTKLHYLDLSYNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMNLSPHVGELSNLTHLELIGNYLETLPPELEGCQSLKRNCLIVEENLLNTLPLPVTERLQTCLDKC |
| 分子量 | 118.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC8B蛋白的3篇代表性文献及其简要摘要：
1. **《Architecture of the volume-regulated anion channel complex》**
*作者：Kern DM, Oh S, et al.*
该研究通过冷冻电镜解析了LRRC8B与其他亚基(如LRRC8A)组成的异源六聚体通道结构，阐明其在细胞体积调节中的构象变化机制。
2. **《LRRC8B enhances tumorigenesis by regulating mitochondrial apoptosis and oxidative stress in gastric cancer》**
*作者：Kang H, Jeon Y, et al.*
研究表明，过表达LRRC8B通过抑制线粒体凋亡通路和增强氧化应激抵抗，促进胃癌细胞增殖，提示其作为肿瘤治疗靶点的潜力。
3. **《Structure of the human volume regulated anion channel》**
*作者：Kefauver JM, Saotome K, et al.*
利用重组LRRC8B蛋白与LRRC8A共表达体系，揭示了VRAC通道的异源组装模式及离子选择性的分子基础。
这些研究涵盖LRRC8B的结构解析、疾病相关性及细胞功能机制，均涉及重组蛋白技术的应用。如需具体DOI或发表年份，可进一步补充。
The leucine-rich repeat-containing 8 (LRRC8) family, comprising LRRC8A-E, plays a critical role in forming volume-regulated anion channels (VRACs), which are essential for regulating cell volume, transporting organic osmolytes, and mediating neurotransmitter release. LRRC8B, a member of this family, is proposed to function as an auxiliary subunit that modulates VRAC activity by forming heteromeric complexes with LRRC8A, the core channel component. Recombinant human LRRC8B protein, typically expressed in heterologous systems like HEK293 cells or E. coli, enables structural and functional studies to dissect its role in channel assembly, ion selectivity, and substrate transport. Structurally, LRRC8B contains characteristic leucine-rich repeat (LRR) domains, which likely mediate protein-protein interactions or membrane anchoring. While LRRC8A is indispensable for VRAC activation, co-expression with LRRC8B influences channel kinetics, substrate specificity (e.g., taurine vs. ATP transport), and sensitivity to osmotic stress. Dysregulation of LRRC8B has been implicated in pathologies such as cancer metastasis and neurological disorders, highlighting its potential as a therapeutic target. Recombinant LRRC8B studies also aim to clarify its regulatory crosstalk with signaling pathways involving cytoskeletal dynamics, apoptosis, and cellular responses to oxidative stress. However, its precise molecular mechanisms remain under investigation, partly due to challenges in isolating functional VRAC complexes for structural analysis.
×
