| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC6 |
| Uniprot No | Q86X45 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-466aa |
| 活性数据 | MGWITEDLIRRNAEHNDCVIFSLEELSLHQQEIERLEHIDKWCRDLKILYLQNNLIGKIENVSKLKKLEYLNLALNNIEKIENLEGCEELAKLDLTVNFIGELSSIKNLQHNIHLKELFLMGNPCASFDHYREFVVATLPQLKWLDGKEIEPSERIKALQDYSVIEPQIREQEKDHCLKRAKLKEEAQRKHQEEDKNEDKRSNAGFDGRWYTDINATLSSLESKDHLQAPDTEEHNTKKLDNSEDDLEFWNKPCLFTPESRLETLRHMEKQRKKQEKLSEKKKKVKPPRTLITEDGKALNVNEPKIDFSLKDNEKQIILDLAVYRYMDTSLIDVDVQPTYVRVMIKGKPFQLVLPAEVKPDSSSAKRSQTTGHLVICMPKVGEVITGGQRAFKSMKTTSDRSREQTNTRSKHMEKLEVDPSKHSFPDVTNIVQEKKHTPRRRPEPKIIPSEEDPTFEDNPEVPPLI |
| 分子量 | 80.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的3篇关于重组人LRRC6蛋白的示例性参考文献(注：文献为模拟示例，建议通过PubMed等学术平台获取真实文献)：
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1. **标题**：**LRRC6 regulates ciliogenesis by mediating intraflagellar transport**
**作者**：Smith A, et al.
**摘要**：研究揭示了重组人LRRC6蛋白通过与IFT-A复合物相互作用，调控纤毛形成的关键机制，证明其在纤毛组装缺陷相关疾病中的潜在病理作用。
2. **标题**：**Cryo-EM structure of human LRRC6 reveals leucine-rich repeat domain assembly**
**作者**：Chen L, et al.
**摘要**：通过冷冻电镜解析了重组LRRC6蛋白的高分辨率结构，阐明其亮氨酸重复结构域的空间排布及潜在蛋白互作界面，为功能研究提供结构基础。
3. **标题**：**LRRC6 deletion causes male infertility via disrupting sperm flagella development**
**作者**：Wang Y, et al.
**摘要**：利用重组LRRC6蛋白进行功能回补实验，证明其缺失导致小鼠精子鞭毛结构异常和运动障碍，提示LRRC6是男性不育症的潜在致病基因。
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**提示**：实际研究中建议通过关键词“recombinant human LRRC6”在Web of Science或PubMed检索最新文献，优先选择近5年高被引研究或权威期刊论文(如JBC、Cell Research)。若需开放获取资源，可关注Nature Communications或eLife等期刊。
**Background of Recombinant Human LRRC6 Protein**
The leucine-rich repeat-containing protein 6 (LRRC6) is a member of the leucine-rich repeat (LRR) superfamily, characterized by tandem repeats of 20–30 amino acid motifs involved in protein-protein interactions. LRRC6 is primarily localized to the cytoplasm and cilia, playing critical roles in ciliogenesis and ciliary function. Studies link LRRC6 mutations to primary ciliary dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance, leading to chronic respiratory infections, infertility, and situs inversus. LRRC6 interacts with proteins like DNAH5 and DYDC2. participating in the assembly of dynein arms, essential for proper ciliary beating.
Recombinant human LRRC6 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to study its structure, interactions, and disease-related mechanisms. Its production enables functional analyses, such as identifying binding partners or elucidating pathways disrupted in ciliopathies. Additionally, recombinant LRRC6 serves as a potential tool for developing therapeutic strategies targeting ciliary dysfunction. Research on LRRC6 also extends to male infertility, as it is expressed in sperm flagella, highlighting its importance in motility. Despite progress, many aspects of LRRC6’s regulatory mechanisms and broader biological roles remain underexplored, necessitating further investigation.
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