| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC4C |
| Uniprot No | Q9HCJ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 活性数据 | MLNKMTLHPQQIMIGPRFNRALFDPLLVVLLALQLLVVAGLVRAQTCPSVCSCSNQFSKVICVRKNLREVPDGISTNTRLLNLHENQIQIIKVNSFKHLRHLEILQLSRNHIRTIEIGAFNGLANLNTLELFDNRLTTIPNGAFVYLSKLKELWLRNNPIESIPSYAFNRIPSLRRLDLGELKRLSYISEGAFEGLSNLRYLNLAMCNLREIPNLTPLIKLDELDLSGNHLSAIRPGSFQGLMHLQKLWMIQSQIQVIERNAFDNLQSLVEINLAHNNLTLLPHDLFTPLHHLERIHLHHNPWNCNCDILWLSWWIKDMAPSNTACCARCNTPPNLKGRYIGELDQNYFTCYAPVIVEPPADLNVTEGMAAELKCRASTSLTSVSWITPNGTVMTHGAYKVRIAVLSDGTLNFTNVTVQDTGMYTCMVSNSVGNTTASATLNVTAATTTPFSYFSTVTVETMEPSQDEARTTDNNVGPTPVVDWETTNVTTSLTPQSTRSTEKTFTIPVTDINSGIPGIDEVMKTTKIIIGCFVAITLMAAVMLVIFYKMRKQHHRQNHHAPTRTVEIINVDDEITGDTPMESHLPMPAIEHEHLNHYNSYKSPFNHTTTVNTINSIHSSVHEPLLIRMNSKDNVQETQI |
| 分子量 | 98.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC4C蛋白的模拟参考文献示例(注：文献信息为虚构，仅供格式参考)：
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1. **"Structural and Functional Analysis of Recombinant Human LRRC4C in Synaptic Adhesion"**
*作者：Kim S. et al.*
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人LRRC4C蛋白，通过免疫共沉淀实验证实其与Netrin-G1的相互作用。进一步功能实验表明，LRRC4C通过LRR结构域介导神经元突触黏附，并促进海马神经元轴突生长。
2. **"High-Yield Expression and Purification of LRRC4C in Mammalian Cells"**
*作者：Zhang Y. et al.*
**摘要**：作者优化了HEK293细胞中重组人LRRC4C的分泌表达条件，利用亲和层析技术获得高纯度蛋白。研究表明，该重组蛋白具有正确折叠的构象，且与神经胶质细胞表面受体具有特异性结合活性。
3. **"LRRC4C Gene Silencing and Its Role in Glioblastoma Invasion"**
*作者：Wang L. et al.*
**摘要**：通过RNA干扰和重组LRRC4C蛋白回补实验，发现该蛋白通过抑制EGFR/ERK信号通路减少胶质瘤细胞迁移，提示其作为肿瘤抑制因子的潜在治疗价值。
4. **"Crystal Structure of the LRRC4C Extracellular Domain Reveals a Novel Leucine-Rich Repeat Assembly"**
*作者：Harrington E.K. et al.*
**摘要**：利用X射线晶体学解析了LRRC4C胞外结构域的3D结构，揭示了其独特的LRR排列模式，并发现其表面带电区域可能参与配体结合的分子机制。
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*注：以上文献为模拟示例，具体研究请以实际数据库检索结果为准。*
Recombinant human LRRC4C protein, also known as Netrin-G ligand-1 (NGL-1), is a member of the leucine-rich repeat (LRR) superfamily. This transmembrane or secreted protein contains multiple LRR domains, which mediate protein-protein interactions and cellular signaling. LRRC4C is predominantly expressed in the nervous system, where it plays critical roles in axon guidance, synaptic organization, and neuronal connectivity by binding to its receptor, Netrin-G1. It is implicated in regulating synaptic plasticity, a foundation for learning and memory processes.
Research highlights its involvement in neurodevelopmental and psychiatric disorders, including schizophrenia and autism spectrum disorders. Altered LRRC4C expression or mutations have been linked to disrupted neural circuitry and synaptic dysfunction. Recombinant LRRC4C, produced via engineered systems like mammalian or bacterial cells, retains the functional domains necessary for binding Netrin-G1 and modulating downstream pathways. This makes it a valuable tool for studying mechanisms of neural development, synaptic adhesion, and disease-related pathways. Additionally, it serves as a potential therapeutic target or biomarker for neurological conditions. Recent studies also explore its role in cancer, particularly in tumor microenvironment interactions, though this area remains under investigation. The protein's structural versatility and functional diversity underscore its significance in both basic neuroscience and translational research. (Word count: 200)
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