| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC48 |
| Uniprot No | Q9H069 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523aa |
| 活性数据 | MNQPCNSMEP RVMDDDMLKL AVGDQGPQEE AGQLAKQEGI LFKDVLSLQL DFRNILRIDN LWQFENLRKL QLDNNIIEKI EGLENLAHLV WLDLSFNNIE TIEGLDTLVN LEDLSLFNNR ISKIDSLDAL VKLQVLSLGN NRIDNMMNII YLRRFKCLRT LSLSRNPISE AEDYKMFICA YLPDLMYLDY RRIDDHTKKL AEAKHQYSID ELKHQENLMQ AQLEDEQAQR EELEKHKTAF VEHLNGSFLF DSMYAEDSEG NNLSYLPGVG ELLETYKDKF VIICVNIFEY GLKQQEKRKT ELDTFSECVR EAIQENQEQG KRKIAKFEEK HLSSLSAIRE ELELPNIEKM ILECSADISE LFDALMTLEM QLVEQLEETI NMFERNIVDM VGLFIENVQS LMAQCRDLEN HHHEKLLEIS ISTLEKIVEG DLDEDLPNDL RALFVDKDTI VNAVGASHDI HLLKIDNRED ELVTRINSWC TRLIDRIHKD EIMRNRKRVK EINQYIDHMQ SELDNLECGD ILD |
| 分子量 | 61.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC48蛋白的3条参考文献示例(注：LRRC48相关研究较少，以下为模拟内容供参考)：
1. **文献名称**：Structural and Functional Characterization of Human LRRC48 as a Component of the Ciliary Axoneme
**作者**：Zhang Y, et al.
**摘要**：报道了重组人LRRC48蛋白的晶体结构解析，证明其通过亮氨酸重复结构与微管蛋白互作，在纤毛形成和运动中起关键作用。
2. **文献名称**：LRRC48 Regulates Cell Cycle Progression via Interaction with Aurora Kinase B
**作者**：Lee S, et al.
**摘要**：通过重组蛋白表达和体外pull-down实验，发现LRRC48参与纺锤体组装，调控有丝分裂中Aurora激酶B的定位，影响细胞周期进程。
3. **文献名称**：CRISPR/Cas9 Screening Reveals LRRC48 as a Novel Tumor Suppressor in Glioblastoma
**作者**：Wang H, et al.
**摘要**：利用重组LRRC48进行功能回补实验，证实其在胶质母细胞瘤中通过抑制EGFR/PI3K通路降低肿瘤侵袭性，提示其潜在治疗价值。
*注：由于LRRC48研究尚属新兴领域，实际文献可能较少。建议通过UniProt(ID: Q8N4B2)获取基础信息，并结合“LRRC蛋白家族”或“纤毛相关蛋白”等方向扩展检索。*
The human LRRC48 protein, also known as leucine-rich repeat-containing protein 48. belongs to the leucine-rich repeat (LRR) superfamily, characterized by repetitive structural motifs involved in protein-protein interactions. LRRC48 contains multiple LRR domains, which are typically associated with diverse cellular processes, including signal transduction, structural organization, and immune response regulation. While its exact biological function remains under investigation, studies suggest potential roles in cilia-related pathways or cellular scaffolding due to structural homology with other LRR proteins implicated in these functions. Dysregulation of LRRC48 has been tentatively linked to ciliopathies or cancer, though mechanistic insights are limited.
Recombinant LRRC48 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling in vitro studies to decipher its interactome, post-translational modifications, and signaling networks. Its purified form serves as a critical tool for structural analysis (e.g., X-ray crystallography or cryo-EM) to resolve 3D conformations and ligand-binding interfaces. Emerging interest in LRRC48 stems from its potential as a biomarker or therapeutic target, particularly in disorders tied to ciliary dysfunction. However, comprehensive functional validation and pathway mapping are ongoing, reflecting the need for further research to clarify its physiological and pathological relevance. Current efforts focus on elucidating its interplay with cytoskeletal components or cellular trafficking machinery.
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