| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRRC20 |
| Uniprot No | Q8TCA0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-184aa |
| 活性数据 | MLKKMGEAVARVARKVNETVESGSDTLDLAECKLVSFPIGIYKVLRNVSGQIHLITLANNELKSLTSKFMTTFSQLRELHLEGNFLHRLPSEVSALQHLKAIDLSRNQFQDFPEQLTALPALETINLEENEIVDVPVEKLAAMPALRSINLRFNPLNAEVRVIAPPLIKFDMLMSPEGARAPLP |
| 分子量 | 46.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRRC20蛋白的3条示例参考文献，包括文献名称、作者及摘要概括：
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**1. "Expression and Functional Characterization of Recombinant Human LRRC20 in Cancer Cell Migration"**
**作者**: Zhang Y., Li X., Chen H.
**摘要**: 本研究成功克隆并在HEK293细胞中表达了重组人LRRC20蛋白，发现其通过调控EGFR信号通路促进肿瘤细胞迁移。蛋白质相互作用实验表明，LRRC20与细胞骨架蛋白相互作用，提示其在癌症转移中的潜在作用。
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**2. "Structural Insights into LRRC20: Role of Leucine-Rich Repeats in Protein-Protein Interactions"**
**作者**: Smith J.R., Kumar S., Yamamoto M.
**摘要**: 通过X射线晶体学解析了LRRC20的胞外结构域，揭示了其富含亮氨酸重复序列(LRR)的构象。该研究提出LRRC20可能通过LRR结构域介导与Toll样受体家族成员的相互作用，为炎症反应的调控机制提供了新视角。
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**3. "Optimization of Soluble Expression and Purification of Recombinant LRRC20 in Mammalian Systems"**
**作者**: Wang L., Park J., Kim T.
**摘要**: 系统比较了哺乳动物表达系统(CHO和HEK293)中重组人LRRC20蛋白的可溶性表达条件，通过优化载体设计和培养参数显著提高了蛋白产量。纯化的LRRC20适用于后续抗体开发及功能研究。
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注：以上文献为示例，实际研究中请根据具体数据库检索可靠来源。若需进一步扩展，可关注LRRC20在疾病模型、信号通路或分子互作领域的实证研究。
The leucine-rich repeat-containing protein 20 (LRRC20) is a member of the leucine-rich repeat (LRR) superfamily, characterized by repeating structural motifs of 20-30 amino acids rich in leucine residues. These proteins typically function in protein-protein interactions, cellular signaling, and structural organization. LRRC20. encoded by the *LRRC20* gene in humans, is a less-studied member of this family, but its conserved LRR domains suggest roles in molecular recognition and interaction networks. Structurally, it likely adopts a horseshoe-shaped fold, enabling versatile binding to ligands, receptors, or other signaling molecules.
Emerging studies implicate LRRC20 in diverse physiological and pathological processes. Bioinformatic analyses associate it with cellular adhesion, immune regulation, and tumorigenesis, though mechanistic details remain limited. Some evidence links LRRC20 overexpression to cancers, including hepatocellular carcinoma and breast cancer, where it may modulate signaling pathways like Wnt/β-catenin or NF-κB. Additionally, its potential involvement in neurological disorders and cilia-related functions has been hypothesized based on homology with other LRR proteins.
Recombinant human LRRC20 protein is engineered for in vitro studies to dissect its molecular interactions, structural properties, and functional roles. Produced via expression systems like *E. coli* or mammalian cells, it often includes tags (e.g., His or GST) for purification and detection. Researchers utilize it to identify binding partners, screen for inhibitors, or study post-translational modifications. Its recombinant form is critical for advancing basic research and exploring therapeutic applications, such as targeted cancer therapies or biomarker development. Further studies are needed to fully elucidate its biological significance and clinical potential.
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