| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRIT3 |
| Uniprot No | Q3SXY7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-522aa |
| 活性数据 | MPLLRTLDLHNNKITSVPNEALRYLKNLAYLDLSSNRLTTLTPDFLENWTHLVSTPSGVLDLSPSRIILGLQDNPWFCDCHISKMIELSKVVDPAIVLLDPLMTCSEPERLTGILFQRAELEHCLKPSVMTSATKIMSALGSNVLLRCDATGFPTPQITWTRSDSSPVNYTVIQESPEEGVRWSIMSLTGISSKDAGDYKCKAKNLAGMSEAVVTVTVLGITTTPIPPDTSERTGDHPEWDVQPGSGRSTSVSSASSYLWSSSFSPTSSFSASTLSPPSTASFSLSPFSSSTVSSTTTLSTSISASTTMANKRSFQLHQGGKRNLKVAKNGSKLPPASTSKKEELALLDQTMLTETNATIENLRVVSETKESVTLMWNMINTTHNSAVTVLYSKYGGKDLLLLNADSSKNQVTIDGLEPGGQYMACVCPKGVPPQKDQCITFSTERVEGDDSQWSLLLVVTSTACVVILPLICFLLYKVCKLQCKSEPFWEDDLAKETYIQFETLFPRSQSVGELWTRSHRDDSEKLLLCSRSSVESQVTFKSEGSRPEYYC |
| 分子量 | 86.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LRIT3蛋白的参考文献示例，涵盖蛋白功能、结构及治疗应用等方向：
1. **标题**：**LRIT3 interactions with presynaptic calcium channels in photoreceptor synapses**
**作者**：Aoki et al. (2016)
**摘要**：研究通过重组人LRIT3蛋白揭示了其与视网膜光感受器突触前钙通道的相互作用，表明LRIT3在视觉信号转导中调控钙稳态，突变可导致先天性夜盲症。
2. **标题**：**Structural insights into the LRIT3-NETO2 complex in synaptic organization**
**作者**：Zenaty et al. (2018)
**摘要**：利用重组LRIT3蛋白进行结构分析，发现其C端结构域与突触后受体NETO2特异性结合，阐释了其在视网膜神经元突触组装中的分子机制。
3. **标题**：**Functional rescue of LRIT3-associated night blindness using recombinant protein therapy in mice**
**作者**：Kubota et al. (2021)
**摘要**：在小鼠模型中验证重组人LRIT3蛋白的基因治疗效果，发现其可恢复视网膜突触功能，为相关遗传性眼病提供潜在治疗策略。
4. **标题**：**Expression and purification of recombinant human LRIT3 for biochemical characterization**
**作者**：Smith et al. (2019)
**摘要**：优化重组人LRIT3蛋白在大肠杆菌中的可溶性表达方案，并通过圆二色谱验证其二级结构，为后续功能研究提供高纯度蛋白。
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*注：上述文献为示例性概括，实际研究需通过学术数据库核实具体信息。*
**Background of Recombinant Human LRIT3 Protein**
LRIT3 (Leucine-Rich Repeat, Immunoglobulin-like, and Transmembrane Domains 3) is a protein encoded by the *LRIT3* gene, predominantly expressed in the retina. It plays a critical role in visual signaling by modulating the function of ON bipolar cells, which are essential for transmitting light-evoked signals from photoreceptors to retinal neurons. Mutations in *LRIT3* are linked to congenital stationary night blindness (CSNB), a non-progressive retinal disorder characterized by impaired dim-light vision.
Recombinant human LRIT3 protein is generated using biotechnological methods, typically through expression in mammalian or bacterial systems, ensuring high purity and bioactivity. Studying this protein aids in understanding its structural and functional roles in retinal circuitry, particularly its interaction with metabotropic glutamate receptors (mGluR6) and transducin-like proteins to regulate synaptic transmission. Recombinant LRIT3 also serves as a tool for developing therapeutic strategies, such as gene therapy or pharmacological interventions, for CSNB and related retinal disorders. Its application extends to disease modeling, antibody development, and mechanistic studies of photoreceptor-bipolar cell communication. Research on LRIT3 continues to unravel its broader implications in retinal health and disease, highlighting its potential as a therapeutic target.
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