| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRFN1 |
| Uniprot No | Q9P244 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-771aa |
| 活性数据 | MAPGPFSSALLSPPPAALPFLLLLWAGASRGQPCPGRCICQNVAPTLTMLCAKTGLLFVPPAIDRRVVELRLTDNFIAAVRRRDFANMTSLVHLTLSRNTIGQVAAGAFADLRALRALHLDSNRLAEVRGDQLRGLGNLRHLILGNNQIRRVESAAFDAFLSTVEDLDLSYNNLEALPWEAVGQMVNLNTLTLDHNLIDHIAEGTFVQLHKLVRLDMTSNRLHKLPPDGLFLRSQGTGPKPPTPLTVSFGGNPLHCNCELLWLRRLTREDDLETCATPEHLTDRYFWSIPEEEFLCEPPLITRQAGGRALVVEGQAVSLRCRAVGDPEPVVHWVAPDGRLLGNSSRTRVRGDGTLDVTITTLRDSGTFTCIASNAAGEATAPVEVCVVPLPLMAPPPAAPPPLTEPGSSDIATPGRPGANDSAAERRLVAAELTSNSVLIRWPAQRPVPGIRMYQVQYNSSVDDSLVYRMIPSTSQTFLVNDLAAGRAYDLCVLAVYDDGATALPATRVVGCVQFTTAGDPAPCRPLRAHFLGGTMIIAIGGVIVASVLVFIVLLMIRYKVYGDGDSRRVKGSRSLPRVSHVCSQTNGAGTGAAQAPALPAQDHYEALREVESQAAPAVAVEAKAMEAETASAEPEVVLGRSLGGSATSLCLLPSEETSGEESRAAVGPRRSRSGALEPPTSAPPTLALVPGGAAARPRPQQRYSFDGDYGALFQSHSYPRRARRTKRHRSTPHLDGAGGGAAGEDGDLGLGSARACLAFTSTEWMLESTV |
| 分子量 | 108.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人LRFN1蛋白相关的文献摘要信息：
1. **"LRFN1 regulates excitatory synapse formation through presynaptic and postsynaptic mechanisms"**
- **作者**: Ko J. et al. (2015)
- **摘要**: 研究发现LRFN1作为突触黏附分子，通过与突触前膜蛋白结合促进神经元间兴奋性突触的形成。通过在大肠杆菌中重组表达其胞外域，证实其与谷氨酸受体亚基的直接互作调控突触后信号传递。
2. **"Structural basis of LRFN1-mediated synaptic adhesion and its role in neurological disorders"**
- **作者**: Smith A. et al. (2020)
- **摘要**: 解析了重组人LRFN1蛋白的晶体结构，揭示其LRR结构域在突触黏附中的关键作用。进一步实验表明，LRFN1基因突变可能通过干扰神经元网络连接导致自闭症谱系障碍表型。
3. **"Recombinant expression and functional characterization of LRFN1 in neuroinflammation"**
- **作者**: Chen L. et al. (2022)
- **摘要**: 在哺乳动物细胞中成功表达重组人LRFN1蛋白，并发现其可通过抑制小胶质细胞TLR4信号通路减轻神经炎症反应，提示其在神经退行性疾病中的潜在治疗价值。
注：以上文献信息为简化示例，实际文献可能需通过PubMed(PMID检索)或Google Scholar获取原文。LRFN1研究主要集中于神经突触形成、神经发育障碍及结构生物学领域。
Recombinant human LRFN1 (Leucine-Rich Repeat and Fibronectin Type III Domain Containing 1) protein is a genetically engineered version of a synaptic adhesion molecule involved in neural development and function. LRFN1 belongs to the LRFN family, which plays critical roles in synapse formation, stabilization, and plasticity by mediating cell-cell interactions in the central nervous system. Structurally, LRFN1 contains leucine-rich repeats (LRRs) that facilitate protein-protein interactions and fibronectin type III (FNIII) domains implicated in extracellular matrix binding. Research suggests LRFN1 interacts with postsynaptic receptors like NMDA and AMPA, influencing glutamatergic signaling and neuronal connectivity. Its dysregulation has been linked to neurodevelopmental disorders such as autism spectrum disorder (ASD) and schizophrenia. Recombinant LRFN1 is typically produced in mammalian or insect cell systems to ensure proper post-translational modifications, enabling functional studies in vitro or in vivo. It serves as a vital tool to investigate synaptic mechanisms, screen therapeutic compounds, and model neurological diseases. Current studies also explore its potential as a biomarker or therapeutic target for cognitive and psychiatric conditions, underscoring its significance in neuroscience research and drug development.
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