| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LPGAT1 |
| Uniprot No | Q92604 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370aa |
| 活性数据 | MAITLEEAPWLGWLLVKALMRFAFMVVNNLVAIPSYICYVIILQPLRVLDSKRFWYIEGIMYKWLLGMVASWGWYAGYTVMEWGEDIKAVSKDEAVMLVNHQATGDVCTLMMCLQDKGLVVAQMMWLMDHIFKYTNFGIVSLVHGDFFIRQGRSYRDQQLLLLKKHLENNYRSRDRKWIVLFPEGGFLRKRRETSQAFAKKNNLPFLTNVTLPRSGATKIILNALVAQQKNGSPAGGDAKELDSKSKGLQWIIDTTIAYPKAEPIDIQTWILGYRKPTVTHVHYRIFPIKDVPLETDDLTTWLYQRFVEKEDLLSHFYETGAFPPSKGHKEAVSREMTLSNLWIFLIQSFAFLSGYMWYNIIQYFYHCLF |
| 分子量 | 69.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LPGAT1蛋白的3篇代表性文献(注：以下内容为示例性虚构，实际文献需通过学术数据库核实)：
1. **文献名称**：*"Functional Characterization of Recombinant Human LPGAT1 in Phospholipid Remodeling"*
**作者**：Smith A, et al.
**摘要**：本研究通过在大肠杆菌中表达重组人LPGAT1蛋白，验证其作为溶血磷脂酰甘油酰基转移酶的活性，证明其在催化磷脂酸(PA)生成中的关键作用，并揭示其底物特异性与细胞膜磷脂稳态的关联。
2. **文献名称**：*"Structural Insights into Human LPGAT1: Implications for Substrate Binding and Inhibitor Design"*
**作者**：Zhang Y, et al.
**摘要**：通过X射线晶体学解析重组人LPGAT1的三维结构，发现其活性位点的保守氨基酸残基参与溶血磷脂酰甘油的酰基转移过程，为靶向LPGAT1的脂代谢疾病治疗提供结构基础。
3. **文献名称**：*"LPGAT1 Deficiency Alters Pulmonary Surfactant Composition and Causes Respiratory Dysfunction in Mice"*
**作者**：Johnson R, et al.
**摘要**：利用重组LPGAT1蛋白进行体外酶活分析，结合基因敲除小鼠模型，揭示LPGAT1在肺表面活性物质磷脂重构中的关键作用，其功能缺失可导致肺泡塌陷和呼吸障碍。
4. **文献名称**：*"Optimization of Recombinant Human LPGAT1 Expression in Insect Cells for High-Throughput Drug Screening"*
**作者**：Lee H, et al.
**摘要**：报道在昆虫杆状病毒表达系统中高效表达并纯化功能性LPGAT1蛋白，建立基于该蛋白的高通量筛选平台，用于发现调节脂代谢的小分子抑制剂。
---
如需真实文献，建议在PubMed或Web of Science中搜索关键词“LPGAT1 recombinant”或“LPGAT1 acyltransferase”。
Lysophosphatidylglycerol acyltransferase 1 (LPGAT1) is a membrane-bound enzyme belonging to the acyltransferase family, primarily localized in the endoplasmic reticulum. It catalyzes the transfer of an acyl group from acyl-CoA to lysophosphatidylglycerol (LPG), generating phosphatidylglycerol (PG), a key phospholipid component of cellular membranes and pulmonary surfactant. PG plays critical roles in maintaining membrane integrity, mitochondrial function, and lipid homeostasis.
Human LPGAT1 is encoded by the *LPGAT1* gene located on chromosome 1p36.11. The protein contains conserved motifs characteristic of the MBOAT (membrane-bound O-acyltransferase) superfamily. Dysregulation of LPGAT1 has been implicated in metabolic disorders, inflammatory responses, and pulmonary pathologies due to its influence on lipid remodeling pathways. Recombinant human LPGAT1 protein is typically expressed in heterologous systems (e.g., *E. coli* or mammalian cell lines) for functional studies. Researchers utilize it to investigate enzyme kinetics, substrate specificity, and inhibitor screening, aiming to develop therapeutic strategies targeting lipid metabolism. Its recombinant form often retains catalytic activity, enabling in vitro reconstitution of PG biosynthesis and structure-function analyses. Current research also explores its role in diseases like nonalcoholic fatty liver disease (NAFLD) and acute respiratory distress syndrome (ARDS), highlighting its biomedical relevance.
×
