| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC642947 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-137aa |
| 活性数据 | MWPGRDCGTGNQGWGLGGALKSDKPCAMGKIVLFCLGPIVSKDQSYLEVCGELWEMDIPGHAPLQPFPHQAFWAPCRLESCFCQLPKQLSLQPQMSVGVMEPPSARILEVHDKNRLLHACLTHPFPRSIWGQNESWC |
| 分子量 | 15.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于重组人LOC642947蛋白可能的研究方向模拟的参考文献示例(注：LOC642947为示例基因编号，实际文献可能需要根据具体数据库核实)：
1. **文献名称**：*Expression and Purification of Recombinant Human LOC642947 Protein in E. coli*
**作者**：Chen L, et al.
**摘要**：本研究成功构建了LOC642947的重组表达载体，利用大肠杆菌系统实现了该蛋白的可溶性表达，并通过镍柱亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**：*Bioinformatic Analysis and Functional Prediction of LOC642947 in Cancer Metastasis*
**作者**：Wang Y, Zhang R.
**摘要**：通过生物信息学分析预测LOC642947蛋白含有多个磷酸化位点，可能与细胞信号转导相关。体外实验表明，该蛋白过表达显著抑制乳腺癌细胞迁移，提示其潜在抑癌作用。
3. **文献名称**：*LOC642947 Structural Characterization and Interaction Partners*
**作者**：Kumar S, et al.
**摘要**：采用圆二色光谱和X射线晶体学解析了LOC642947的二级结构及三维构象，并利用免疫共沉淀技术筛选出其与HSP90的相互作用，提示其可能在蛋白质折叠通路中发挥作用。
4. **文献名称**：*LOC642947 as a Novel Biomarker in Neurodegenerative Diseases*
**作者**：Müller T, et al.
**摘要**：通过阿尔茨海默症患者脑组织样本分析，发现LOC642947表达水平显著下调，且与Aβ蛋白沉积呈负相关，提示其可能参与神经退行性病变的调控机制。
**注意**：以上文献为示例，实际研究中LOC642947可能对应不同命名或尚无明确功能报道，建议通过NCBI PubMed或UniProt数据库查询最新进展。
**Background of Recombinant Human LOC642947 Protein**
LOC642947 is a provisional gene identifier assigned to a hypothetical human protein with limited functional characterization. Currently, its biological role remains poorly understood, and it lacks an official gene symbol or comprehensive annotation in major databases. The protein is predicted to be encoded by an open reading frame (ORF) identified through genomic sequencing, but its expression, regulation, and physiological relevance are yet to be experimentally validated.
Recombinant LOC642947 protein is artificially produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to investigate potential biochemical properties, interactions, and pathways. Structural predictions suggest it may contain conserved domains linked to enzymatic activity or signaling, but these require confirmation.
Interest in LOC642947 stems from its possible involvement in cellular processes inferred from sequence homology with characterized proteins. However, the absence of targeted studies limits definitive conclusions. Research efforts focus on clarifying its role in health and disease, particularly its expression patterns in tissues and implications in pathologies like cancer or metabolic disorders.
Overall, LOC642947 represents an underexplored protein with unresolved significance. Its recombinant production is a critical step toward unlocking mechanistic insights, paving the way for applications in drug discovery, biomarker development, or molecular tool design. Further studies are essential to validate its function and therapeutic potential.
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