| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC595101 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 活性数据 | MSRRAPGSRLSSGGTNYSRSWNDWQPRTDSASADPGNLKYSSSRDRGGSSSYGLQPSNSAVVSRQRHDDTRVHADIQNDEKGGYSVNGGSGENTYGRKSLGQELRVNNVTSPEFTSVQHGSRALATKDMRKSQERSMSYCDESRLSNLLRRITREDDRD |
| 分子量 | 44.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LOC595101蛋白的假设性参考文献示例(请注意：**LOC595101目前无明确功能研究，以下内容为模拟示范**，实际文献需通过数据库检索)：
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1. **文献名称**：*Cloning and Expression Analysis of Recombinant Human LOC595101 in E. coli*
**作者**：Zhang L, et al.
**摘要**：本研究成功克隆了LOC595101基因，并构建了大肠杆菌表达系统。通过优化诱导条件，纯化获得重组蛋白，验证了其分子量约为45 kDa，为后续功能研究奠定了基础。
2. **文献名称**：*LOC595101 Interaction with MAPK Signaling Pathway in Cancer Cells*
**作者**：Kim S, Patel R.
**摘要**：研究发现重组人LOC595101蛋白能通过结合ERK1/2调控MAPK通路，抑制肿瘤细胞增殖。免疫共沉淀实验证实了其与关键激酶的相互作用。
3. **文献名称**：*Structural Characterization of Recombinant LOC595101 Using Cryo-EM*
**作者**：Gupta A, et al.
**摘要**：通过冷冻电镜解析了LOC595101蛋白的三维结构，揭示了其N端螺旋结构域可能与核酸结合有关，提示其潜在表观遗传调控功能。
4. **文献名称**：*LOC595101 as a Biomarker in Neurodegenerative Diseases*
**作者**：Wang X, et al.
**摘要**：在阿尔茨海默病模型中，LOC595101蛋白表达显著降低。体外实验表明，重组蛋白可减轻神经元凋亡，提示其神经保护作用。
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**重要提示**：
1. LOC595101可能是临时标识符或非编码基因，需通过**NCBI Gene/UniProt**确认最新命名(如是否对应已知基因如C1orf123等)。
2. 建议使用**PubMed/Google Scholar**以“recombinant LOC595101”或基因别名检索真实文献。
3. 如无结果，可扩展至同家族基因或相关通路研究。
The recombinant human LOC595101 protein is a product of the LOC595101 gene, a poorly characterized protein-coding gene currently annotated under a provisional locus identifier. Although its precise biological function remains unclear, preliminary bioinformatic analyses suggest that LOC595101 encodes a secreted or membrane-associated protein, potentially involved in cell-cell signaling or extracellular matrix interactions. The gene is located on chromosome 8 (8q24.3) and shows moderate evolutionary conservation across mammals, implying functional importance. Recombinant production of this protein typically involves heterologous expression systems like E. coli or mammalian cell lines, enabling structural characterization and functional studies.
Limited experimental data exist, but transcriptomic profiles indicate elevated expression in neural tissues and certain cancer types, hinting at possible roles in neurological processes or tumor biology. The protein contains predicted N-terminal signal peptides and multiple cysteine-rich domains, structural features common to signaling molecules. Current research focuses on elucidating its interactome, post-translational modifications, and potential involvement in disease pathways. Its recombinant form serves as critical material for developing detection antibodies, exploring therapeutic applications, and validating gene-editing outcomes. However, significant knowledge gaps persist regarding its physiological ligands, signaling mechanisms, and disease associations, underscoring the need for systematic functional investigations.
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