| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC554223 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 活性数据 | MDWGGPALGIPHLCRVSLLPLPTCVGSFFLGTHRAAPVLTPIECRVSREANQCSRGPGSKVPTHPPGLRFFPVAEDGVMAPRTLLLLLSGALVLTQTWAGFHSLRYFHTTMSRPGRADPRFLSVGDVDDTQCVRLDSDATSPRMEPRAPWMEQEGPEYWEEETGTAKAKAQFYRVNLRTLSGYYNQSEA |
| 分子量 | 47.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为构造的3-4条虚拟参考文献示例，供参考：
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1. **文献名称**: *Functional Analysis of Recombinant Human LOC554223 Protein in Cell Proliferation*
**作者**: Zhang, L.; Wang, X. et al.
**摘要**: 本研究在大肠杆菌中成功表达重组人LOC554223蛋白，发现其通过调控ERK信号通路促进癌细胞增殖，敲低实验显示细胞周期阻滞在G1期，提示其在肿瘤中的潜在作用。
2. **文献名称**: *LOC554223 as a Novel Biomarker in Autoimmune Disorders: Recombinant Protein Studies*
**作者**: Kim, S.; Tanaka, H. et al.
**摘要**: 利用HEK293细胞表达纯化的LOC554223蛋白，发现其与IL-17存在相互作用，在类风湿性关节炎患者血清中表达上调，可能成为自身免疫疾病的诊断标记物。
3. **文献名称**: *Structural Characterization and Apoptotic Role of Recombinant LOC554223*
**作者**: Müller, R.; Silva, J. et al.
**摘要**: 通过X射线晶体学解析LOC554223的三维结构，发现其BH3结构域可与Bcl-2家族蛋白结合，诱导线粒体依赖性凋亡，为靶向癌症治疗提供新方向。
4. **文献名称**: *LOC554223 Recombinant Protein Attenuates Fibrosis in Mouse Models*
**作者**: Chen, Y.; Gonzalez, A. et al.
**摘要**: 在小鼠肺纤维化模型中注射重组LOC554223.显著降低TGF-β1和胶原沉积，机制涉及抑制Smad3磷酸化，提示其抗纤维化治疗潜力。
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*注：LOC554223为虚构/暂定基因符号，实际文献需根据具体基因命名(如已更名)检索。若需真实文献，请核查基因最新符号及数据库(如PubMed)。*
Recombinant human LOC554223 protein is a genetically engineered protein derived from the human gene LOC554223. a locus currently annotated as a long non-coding RNA (lncRNA) gene in public genomic databases (e.g., NCBI, Ensembl). Despite its classification, emerging studies suggest potential small open reading frames (sORFs) within LOC554223 transcripts, hinting at possible protein-coding capacity or regulatory roles in cellular processes. The functional relevance of LOC554223 remains poorly characterized, though bioinformatic analyses associate it with pathways involving cell cycle regulation, stress response, or tumorigenesis. Recombinant expression of LOC554223 allows researchers to probe its biochemical properties, interactome, and mechanistic contributions to diseases like cancer, where dysregulation of similar lncRNAs has been documented. Production typically involves cloning synthesized cDNA sequences into prokaryotic or eukaryotic expression systems, followed by purification using affinity tags. Characterization efforts focus on validating its expression in native tissues, identifying binding partners, and assessing its role in signaling cascades or epigenetic modulation. While still in exploratory stages, studying recombinant LOC554223 protein may uncover novel therapeutic targets or biomarkers, bridging gaps between non-coding genomic regions and functional proteomics. Current research remains limited, emphasizing the need for functional validation to clarify its physiological and pathological significance.
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