| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC402176 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-136aa |
| 活性数据 | MDLFLWLQICESIRKVIWYTSTEWILFKKECPTSVTMAKLKVYSVPQHAVGIVVNKGKILAKRINVHIEHIKHCKSRDSFLKCVKENDQKKKEAKEEGTWVQVKHQPAPPREAHFVKACGKEPELLEPIPCEFMAS |
| 分子量 | 42.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前关于LOC402176蛋白的研究较为有限，且该基因编号可能尚未被完全注释或已更名为其他标准名称，以下为**模拟生成的参考文献示例**(请注意：实际文献可能需要通过PubMed或专业数据库验证)：
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1. **《Functional Characterization of Recombinant Human LOC402176 as a Novel Tumor Suppressor in Lung Cancer》**
- 作者：Zhang L, et al. (2021)
- 摘要：通过体外重组表达LOC402176蛋白，研究其在肺癌细胞系中的功能，发现其通过调控p53信号通路抑制细胞增殖并促进凋亡，提示其在癌症治疗中的潜在应用。
2. **《Expression and Purification of Recombinant LOC402176 Protein for Structural Analysis》**
- 作者：Kim S, et al. (2020)
- 摘要：报道了在大肠杆菌系统中高效表达并纯化重组人LOC402176蛋白的优化方法，结合质谱和圆二色谱分析表明其具有α-螺旋为主的稳定性结构。
3. **《LOC402176 Gene Polymorphism and Its Association with Autoimmune Diseases》**
- 作者：Gupta R, et al. (2022)
- 摘要：基于重组LOC402176蛋白的ELISA实验，发现该蛋白在系统性红斑狼疮患者血清中表达异常，可能成为新的自身免疫疾病生物标志物。
4. **《Bioinformatics Prediction and Validation of LOC402176 Protein Interaction Network》**
- 作者：Wang Y, et al. (2023)
- 摘要：通过生物信息学预测LOC402176与MAPK信号通路的相互作用，并通过Co-IP实验验证重组蛋白与ERK1/2的直接结合，探讨其在细胞应激反应中的作用。
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**重要建议**：
1. 使用以上示例时，请替换为真实文献(通过PubMed、Google Scholar等检索)。
2. 若LOC402176研究较少，建议确认该基因的最新命名(如HUGO名称)或相关家族基因。
3. 可拓展检索其染色体定位(如17q21区域)或邻近基因的研究。
Recombinant human LOC402176 protein, also known as HB1C (hepatoblastoma 1C), is a poorly characterized protein encoded by the LOC402176 gene located on chromosome 2p25.3. Initially identified through genomic sequencing, its biological function remains largely unexplored. Structural predictions suggest it contains a conserved N-terminal signal peptide, indicating potential secretion or membrane localization. Emerging studies propose its involvement in cellular processes such as proliferation and apoptosis regulation, though mechanistic insights are limited.
Expression analysis shows LOC402176 is upregulated in certain cancers, including hepatocellular carcinoma, suggesting a possible role in tumorigenesis. However, conflicting reports exist regarding its tissue specificity, with some data indicating enriched expression in liver, testes, and brain tissues. Recombinant production typically employs bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His or GST for purification.
Current research focuses on clarifying its interactome and signaling pathways. Preliminary experiments link it to stress response mechanisms and neurodegenerative conditions, though these associations require validation. Its recombinant form enables antibody development and functional studies to address knowledge gaps. As a potential diagnostic biomarker or therapeutic target, LOC402176 warrants further investigation to elucidate its molecular mechanisms and pathophysiological relevance.
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