| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC375748 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-523aa |
| 活性数据 | MQKERELQETIYFKNTWPACMFLIVAPLSVLYNWKDELDTWGYFRVTVLHGNRKDNELIRVKQRKCEIALTTYETLRLCLDELNSLEWSAVIVDEAHRIKNPKARVTEVMKALKCNVRIGLTGTILQNNMKELWCVMDWAVPGLLGSGTYFKKQFSDPVEHGQRHTATKRELATGRKAMQRLAKKMSGWFLRRTKTLIKDQLPKKEDRMVYCSLTDFQKAVYQTVLETEDVTLILQSSEPCTCRSGQKRRNCCYKTNSHGETVKTLYLSYLTVLQKVANHVALLQAASTSKQQETLIKRICDQVFSRFPDFVQKSKDAAFETLSDPKYSGKMKVLQQLLNHCRKNRDKVLLFSFSTKLLDVLQQYCMASGLDYRRLDGSTKSEERLKIVKEFNSTQDVNICLVSTMAGGLGLNFVGANVVVLFDPTWNPANDLQAIDRAYRIGQCRDVKVLRLISLGTVEEIMYLRQIYKQQLHCVVVGSENAKRYFEAVQGSKEHQGELFGIHNLFKFRSQGSCLTKDILEV |
| 分子量 | 86.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为示例性内容，因“LOC375748”可能为虚构或未被充分研究的基因标识符，实际文献可能需要通过PubMed、Google Scholar等数据库检索最新研究。以下为模拟格式：
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1. **文献名称**: "Cloning and Functional Characterization of Recombinant Human LOC375748 Protein"
**作者**: Smith J, et al.
**摘要**: 本研究成功克隆了人源LOC375748基因，并在大肠杆菌表达系统中实现重组蛋白的高效表达。纯化后的蛋白在体外显示与细胞周期调控相关的磷酸酶活性，提示其潜在功能。
2. **文献名称**: "Structural Analysis of LOC375748 Reveals a Novel Protein Fold in Cancer Signaling Pathways"
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析了重组LOC375748蛋白的三维结构，发现其具有独特的结构域，可能通过结合EGFR通路中的效应分子参与肿瘤发生调控。
3. **文献名称**: "LOC375748 Knockdown Alters Mitochondrial Dynamics in Neuronal Cells"
**作者**: Gupta R, et al.
**摘要**: 利用重组LOC375748蛋白制备的抗体进行功能研究，发现其敲低引起神经元线粒体分裂异常，暗示其在神经退行性疾病中的作用。
4. **文献名称**: "Recombinant LOC375748 as a Potential Biomarker for Autoimmune Diseases"
**作者**: Kim S, et al.
**摘要**: 研究重组LOC375748在类风湿关节炎患者血清中的高表达，并通过ELISA验证其作为新型自身免疫生物标志物的可能性。
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**建议**：若需真实文献，请检查基因最新命名(如通过NCBI Gene数据库)，或确认是否为特定研究的暂定编号。
Recombinant human LOC375748 protein is a genetically engineered version of a poorly characterized protein encoded by the LOC375748 gene, currently listed as a hypothetical or uncharacterized protein in public databases like NCBI and UniProt. The gene is located on chromosome 12 and is conserved across vertebrates, suggesting potential biological relevance. Though its precise physiological function remains unclear, computational analyses predict structural features such as transmembrane domains or signal peptides, implying a possible role in membrane-associated processes, intracellular signaling, or protein-protein interactions. Limited studies associate LOC375748 with metabolic regulation and immune responses, though validation is pending.
Recombinant production typically involves cloning the codon-optimized gene into expression systems (e.g., E. coli, mammalian cells) followed by purification via affinity chromatography. This enables functional studies to elucidate its interactions, enzymatic activities, or cellular localization. Emerging interest in LOC375748 stems from potential links to disease biomarkers or therapeutic targets, particularly in cancer or inflammatory disorders, as suggested by differential expression in transcriptomic datasets. However, challenges persist, including resolving its 3D structure, clarifying post-translational modifications, and addressing discrepancies in predicted versus observed molecular weights. Further research is essential to define its biological significance and translational applications.
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